Plasma ghrelin levels in lean and obese humans and the effect of glucose on ghrelin secretion.
Plasma ghrelin levels in lean and obese humans and the effect of glucose on ghrelin secretion.
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DOI：
10.1210/jcem.87.1.8129
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发表时间：
2002
期刊：
影响因子：
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通讯作者：
Tomomi Shiiya;M. Nakazato;M. Mizuta;Y. Date;M. S. Mondal;Muneki Tanaka;S. Nozoe;H. Hosoda;K. Kangawa;S. Matsukura
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文献类型：
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作者：
Tomomi Shiiya;M. Nakazato;M. Mizuta;Y. Date;M. S. Mondal;Muneki Tanaka;S. Nozoe;H. Hosoda;K. Kangawa;S. Matsukura
Ghrelin是从人和大鼠胃中分离出来的一种新的GH释放肽，能刺激食物的摄取和GH的分泌。我们用放射免疫法测定了单纯性肥胖、神经性厌食症和2型糖尿病患者的血浆Ghrelin浓度。我们还研究了血浆Ghrelin对葡萄糖负荷和膳食摄入量的反应，并获得了人类循环Ghrelin的24小时曲线。单纯性肥胖和神经性厌食症患者的血浆Ghrelin浓度分别低于正常体重健康人和高于正常体重健康人。在2型糖尿病患者中，肥胖患者的空腹血浆Ghrelin浓度低于正常体重患者，而消瘦患者的空腹血浆Ghrelin浓度高于正常体重患者。在非糖尿病和糖尿病患者中，空腹血浆Ghrelin浓度与体重指数呈负相关。口服和静脉注射葡萄糖后，正常人的血浆Ghrelin浓度显著降低；糖尿病患者在餐后耐量试验后也观察到类似的反应，餐后达到基础水平的69%的最低点。循环血浆Ghrelin呈昼夜变化，餐前升高，餐后下降，0200h达高峰，表明营养状态是血浆Ghrelin的决定因素。Ghrelin的分泌在负能量平衡条件下上调，在正能量平衡条件下下调。这些发现表明，Ghrelin参与了摄食行为和能量平衡的调节。
Ghrelin, a novel GH-releasing peptide isolated from human and rat stomach, stimulates food intake and GH secretion. We determined plasma ghrelin concentrations in patients with simple obesity, anorexia nervosa, and type 2 diabetes mellitus by RIA. We also studied plasma ghrelin responses to glucose load and meal intake and obtained a 24-h profile of circulating ghrelin in humans. Plasma ghrelin concentrations in patients with simple obesity and anorexia nervosa were lower and higher, respectively, than those of healthy subjects with normal body weight. Among those with type 2 diabetes mellitus, obese patients had lower and lean patients higher fasting plasma ghrelin concentrations than normal-weight patients. Fasting plasma ghrelin concentration was negatively correlated with body mass index in both nondiabetic and diabetic patients. Plasma ghrelin concentrations of normal subjects decreased significantly after oral and iv glucose administration; a similar response was also observed in diabetic patients after a meal tolerance test, reaching a nadir of 69% of the basal level after the meal. Circulating plasma ghrelin showed a diurnal pattern with preprandial increases, postprandial decreases, and a maximum peak at 0200 h. This study demonstrates that nutritional state is a determinant of plasma ghrelin in humans. Ghrelin secretion is up-regulated under conditions of negative energy balance and down-regulated in the setting of positive energy balance. These findings suggest the involvement of ghrelin in the regulation of feeding behavior and energy homeostasis.