Large-scale transposon mutagenesis of Photobactetium profundum SS9 reveals new genetic loci important for growth at low temperature and high pressure
Large-scale transposon mutagenesis of Photobactetium profundum SS9 reveals new genetic loci important for growth at low temperature and high pressure
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DOI：
10.1128/jb.01176-07
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发表时间：
2008-03-01
影响因子：
3.2
通讯作者：
Bartlett, Douglas H.
中科院分区：
文献类型：
--
作者：
Lauro, Federico M.;Tran, Khiem;Bartlett, Douglas H.
适应于压温生长的微生物在相对恒定的低温和高压下的生物圈中占主导地位，但适应的遗传基础在很大程度上是未知的。在这里，我们报告使用转座子诱变与深海细菌发光杆菌菌株SS 9分离几十个突变株，其生长在低温下受损和/或其生长作为静水压力的函数被改变。在许多情况下，基因突变-生长表型关系通过互补分析来验证。与温度敏感性相关的基因座的最大部分参与细胞被膜的生物合成，特别是胞外多糖的生物合成。与压力敏感性相关的基因座的最大部分涉及染色体结构和功能。核糖体组装和功能的基因被发现对低温和高压生长都很重要。同样地，对温度的适应和对压力的适应都受到许多感觉和调节位点突变的影响，这表明信号转导机制在适应任一物理参数中的重要性。这些分析是首次对深海微生物低温或高压生长所需的基因进行全面分析。
Microorganisms adapted to piezopsychrophilic growth dominate the majority of the biosphere that is at relatively constant low temperatures and high pressures, but the genetic bases for the adaptations are largely unknown. Here we report the use of transposon mutagenesis with the deep-sea bacterium Photobacterium profundum strain SS9 to isolate dozens of mutant strains whose growth is impaired at low temperature and/or whose growth is altered as a function of hydrostatic pressure. In many cases the gene mutation-growth phenotype relationship was verified by complementation analysis. The largest fraction of loci associated with temperature sensitivity were involved in the biosynthesis of the cell envelope, in particular the biosynthesis of extracellular polysaccharide. The largest fraction of loci associated with pressure sensitivity were involved in chromosomal structure and function. Genes for ribosome assembly and function were found to be important for both low-temperature and high-pressure growth. Likewise, both adaptation to temperature and adaptation to pressure were affected by mutations in a number of sensory and regulatory loci, suggesting the importance of signal transduction mechanisms in adaptation to either physical parameter. These analyses were the first global analyses of genes conditionally required for low-temperature or high-pressure growth in a deep-sea microorganism.