Analysis of cellular proteome alterations in porcine alveolar macrophage cells infected with 2009 (H1N1) and classical swine H1N1 influenza viruses.
Analysis of cellular proteome alterations in porcine alveolar macrophage cells infected with 2009 (H1N1) and classical swine H1N1 influenza viruses.
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DOI：
10.1016/j.jprot.2011.12.012
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发表时间：
2012-03
影响因子：
3.3
通讯作者：
Jiping Zhu;W. Zou;Guangmin Jia;Hongbo Zhou;Yong Hu;Mengyun Peng;Huanchun Chen;M. Jin
中科院分区：
文献类型：
--
作者：
Jiping Zhu;W. Zou;Guangmin Jia;Hongbo Zhou;Yong Hu;Mengyun Peng;Huanchun Chen;M. Jin
H1N1/2009流感病毒有可能导致人类大流行，已有零星的人与猪传播病例报告。在本研究中，从猪身上分离到两种流感病毒。系统进化分析表明，A/Sine/南昌/F9/2010(H1N1)(F9/10)毒株与H1N1/2009大流行毒株高度同源，A/Sine/广东/34/2006(H1N1)(34/06)毒株是一株典型的猪流感病毒。用蛋白质组学方法研究了F9/10和34/06病毒感染猪肺泡巨噬细胞(PAM)后不同时间蛋白表达的变化。利用双向凝胶电泳法结合MALDI-TOF MS/MS，我们鉴定了13个上调的蛋白质点和21个下调的蛋白质点，包括细胞骨架蛋白、细胞信号转导蛋白、分子生物合成蛋白和热休克蛋白。感染细胞中最显著的变化与分子生物合成蛋白和热休克蛋白有关。我们分析了F9/10和34/06病毒在体内和体外的生物学特性。F9/10病毒对PAM细胞和小鼠的致病性强于34/06病毒。这项研究为H1N1/2009大流行的生物学特征、潜在的毒力变化和跨物种传播机制提供了见解。
The H1N1/2009 influenza virus has the potential to cause a human pandemic, and sporadic cases of human-to-pig transmission have been reported. In this study, two influenza viruses were isolated from pigs. A phylogenetic analysis showed that the A/swine/NanChang/F9/2010(H1N1) (F9/10) strain shared a high degree of homology with the pandemic H1N1/2009 virus, and A/swine/GuangDong/34/2006 (H1N1) (34/06) strains was a classical swine influenza virus. A proteomic analysis was performed to investigate possible alterations of protein expression in porcine alveolar macrophage (PAM) cells infected by the F9/10 and 34/06 viruses over different time courses. Using 2-DE in association with MALDI-TOF MS/MS, we identified 13 up-regulated and 21 down-regulated protein spots, including cytoskeleton proteins, cellular signal transduction proteins, molecular biosynthesis proteins and heat shock proteins. The most significant changes in the infected cells were associated with molecular biosynthesis proteins and heat shock proteins. We analysed the biological characteristics of the F9/10 and 34/06 viruses in vivo and in vitro. The F9/10 virus showed greater pathogenicity than the 34/06 virus in PAM cells and mice. This study provides insights into the biologic characteristics, potential virulence alteration and cross-species transmission mechanisms of the pandemic H1N1/2009.