CHARACTERISTICS OF INVASION OF HEP-2 CELLS BY PROVIDENCIA-ALCALIFACIENS
CHARACTERISTICS OF INVASION OF HEP-2 CELLS BY PROVIDENCIA-ALCALIFACIENS
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DOI：
10.1099/00222615-42-3-186
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发表时间：
1995-03-01
影响因子：
3
通讯作者：
FARUQUE, ASG
中科院分区：
文献类型：
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作者：
ALBERT, MJ;ANSARUZZAMAN, M;FARUQUE, ASG
之前对腹泻粪便中三种分离株的研究表明，产碱普罗威登斯菌是一种侵入性肠道病原体，也会导致受感染细胞中的肌动蛋白凝结。这些发现在本研究中得到扩展，进一步使用 14 种腹泻粪便分离的产碱假单胞菌和 HEp-2 细胞单层进行侵袭测定。对两个选定分离株的入侵特征的研究表明，产碱假单胞菌需要在 37 摄氏度下预先生长才能更好地入侵。侵袭和肌动蛋白凝结被抑制微丝形成的试剂抑制，但不能被抑制受体介导的内吞作用、微管形成、内体酸化或受体再循环的试剂抑制。在含有庆大霉素的培养基中维持的 HEp-2 细胞单层的时程测定中，产碱假单胞菌在侵入细胞后显示出小程度的增殖，但在 24 小时内无法恢复活细菌，尽管在此期间保留了细胞单层的完整性。
Previous studies with three isolates from diarrhoeal stools suggested that Providencia alcalifaciens is an invasive enteric pathogen that also causes actin condensation in infected cells. These findings were extended in the present study with a further 14 diarrhoeal stool isolates of P. alcalifaciens and HEp-2 cell monolayers for invasion assays. Studies on invasion characteristics with two selected isolates suggested that P. alcalifaciens required prior growth at 37 degrees C for better invasion. Invasion and actin condensation were inhibited by an agent that inhibits microfilament formation, but not by agents that inhibit receptor-mediated endocytosis, microtubule formation, endosome acidification or receptor recycling. In time-course assays with HEp-2 cell monolayers maintained in medium containing gentamicin, P. alcalifaciens showed a small degree of multiplication after invasion of the cells, but viable bacteria could not be recovered over a 24-h period although the integrity of the cell monolayer was preserved during this period.