Establishment of rules for interpreting ultraviolet autofluorescence microscopy images for noninvasive detection of Barrett's esophagus and dysplasia
Establishment of rules for interpreting ultraviolet autofluorescence microscopy images for noninvasive detection of Barrett's esophagus and dysplasia
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DOI：
10.1117/1.jbo.17.1.016013
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发表时间：
2012-01-01
影响因子：
3.5
通讯作者：
Demos, Stavros G.
中科院分区：
文献类型：
--
作者：
Lin, Bevin;Urayama, Shiro;Demos, Stavros G.
利用离体人体标本探索了紫外线（UV）激发下自体荧光（AF）显微镜的诊断潜力。目的是建立对应于食管正常和异常组织学的光学模式（解释规则），从早期良性变化（巴雷特食管）到随后的发育异常变化和向癌的进展。这是通过开发按疾病进展分类的图像库来实现的。我们考虑了疾病的形态学变化，因为它们与病理标本的组织学诊断以及正常食管、近端胃和小肠组织的对照样品进行了比较。我们的实验结果表明，UV AF显微镜可以提供实时组织学信息，用于可视化组织微观结构的变化，这些变化目前使用传统的内窥镜方法无法检测到。(c)2012年，美国光电仪器工程师学会（SPIE）。[DOI：10.1117/1.JBO.17.1.016013]
The diagnostic potential of autofluorescence (AF) microscopy under ultraviolet (UV) excitation is explored using ex vivo human specimens. The aim is to establish optical patterns (the rules for interpretation) that correspond to normal and abnormal histologies of the esophagus, spanning from early benign modifications (Barrett's esophagus) to subsequent dysplastic change and progression toward carcinoma. This was achieved by developing an image library categorized by disease progression. We considered morphological changes of disease as they are compared with histological diagnosis of the pathological specimen, as well as control samples of normal esophagus, proximal stomach, and small intestine tissue. Our experimental results indicate that UV AF microscopy could provide real-time histological information for visualizing changes in tissue microstructure that are currently undetectable using conventional endoscopic methods. (c) 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.JBO.17.1.016013]