Complement Activation Is Involved in Renal Damage in Human Antineutrophil Cytoplasmic Autoantibody Associated Pauci-Immune Vasculitis
Complement Activation Is Involved in Renal Damage in Human Antineutrophil Cytoplasmic Autoantibody Associated Pauci-Immune Vasculitis
复制标题
补体激活参与人抗中性粒细胞胞质自身抗体相关寡免疫性血管炎的肾损伤
DOI：
10.1007/s10875-008-9268-2
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发表时间：
2009-05-01
影响因子：
9.1
通讯作者：
Zhao, Ming-hui
中科院分区：
文献类型：
--
作者：
Xing, Guang-qun;Chen, Min;Zhao, Ming-hui
本研究旨在探讨髓过氧化物酶（MPO）-抗中性粒细胞胞浆自身抗体（ANCA）相关少免疫性血管炎患者肾活检标本中补体激活的证据。7名MPO-ANCA阳性寡免疫坏死性坏死性新月体肾炎患者的肾活检标本 (NCGN)采用免疫组织化学和免疫荧光法检测膜攻击复合物(MAC)、C3d、C4d、甘露糖结合凝集素(MBL)、B因子和P因子的染色。 7例微小病变（MCD）患者的肾组织和2例正常肾组织作为对照。 寡免疫AAV患者的肾小球和活动性血管炎的小血管中可检测到MAC、C3d、因子B和因子P，而MCD患者和正常肾组织中不检测或很少检测到MAC、C3d、因子B和因子P。 C3d 和因子 B 与 MAC 共定位，因子 P 与 C3d 共定位。 AAV患者中未检测到MBL和C4d。补体系统的旁路途径参与了人寡免疫AAV的肾损害。
This study was to investigate the evidence for complement activation in renal biopsy specimens of patients with myeloperoxidase (MPO)-antineutrophil cytoplasmic autoantibody (ANCA)-associated pauci-immune vasculitis.Renal biopsy specimens from seven patients with MPO-ANCA positive pauci-immune necrotizing crescentic glomerulonephritis (NCGN) were used to detect the staining of membrane attack complex (MAC), C3d, C4d, mannose-binding lectin (MBL), factor B and factor P using immunohistochemistry and immunofluorescence. Renal tissue from seven patients with minimal change disease (MCD) and two normal renal tissue were used as controls.MAC, C3d, factor B and factor P could be detected in glomeruli and small blood vessels with active vasculitis of patients with pauci-immune AAV, but not or scarcely in patients with MCD and in normal renal tissue. C3d and factor B co-localized with MAC, factor P colocalized with C3d. MBL and C4d were not detected in patients with AAV.The alternative pathway of the complement system is involved in renal damage of human pauci-immune AAV.