Studies in vitro on single beating rat-heart cells X. The effect of linoleic and palmitic acids on beating and mitochondrial phosphorylation
Studies in vitro on single beating rat-heart cells X. The effect of linoleic and palmitic acids on beating and mitochondrial phosphorylation
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大鼠心脏细胞单次跳动的体外研究十、亚油酸和棕榈酸对跳动和线粒体磷酸化的影响
DOI：
10.1016/0005-2728(67)90030-8
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发表时间：
1967
期刊：
影响因子：
--
通讯作者：
J. Mead
中科院分区：
文献类型：
--
作者：
L. Gerschenson;I. Harary;J. Mead
1. 在缺脂培养基中培养的跳动的大鼠心脏细胞显示： (a) 缺乏生长； (b) 减少并随后停止殴打； (c) 氧化磷酸化受损和呼吸控制降低； (d) 气相色谱分析显示脂肪酸缺乏的模式。2．添加白蛋白结合的亚油酸或花生四烯酸部分或完全阻止了脂质缺乏对线粒体功能和脂肪酸组成的影响，但没有促进生长，也没有改善跳动。3．补充白蛋白结合棕榈酸的培养基维持跳动，但不促进生长，也不影响线粒体功能的损伤。
1. Beating rat-heart cells cultured in a lipid-deficient medium showed: (a) lack of growth; (b) decrease and later a cessation of the beating; (c) impairment of oxidative phosphorylation and a decrease in the respiratory control; (d) a pattern of fatty acid deficiency as shown by the analysis using gas-liquid chromatography.2. The addition of albumin-bound linoleic acid or arachidonic acids prevented partially or totally the effects of the lipid deficiency on the mitochondrial function and the fatty acid composition, but did not promote growth and did not improve the beating.3. Medium supplemented with albumin-bound palmitic acid maintained the beating, but did not promote growth and did not affect the impairment of the mitochondrial function.