The proton‐translocating ATPase of Escherichia coli
The proton‐translocating ATPase of Escherichia coli
复制标题
大肠杆菌的质子转位 ATP 酶
DOI：
10.1016/0014-5793(74)80880-x
复制
发表时间：
1974
期刊：
影响因子：
3.5
通讯作者：
P. Mitchell
中科院分区：
文献类型：
--
作者：
I. West;P. Mitchell
Mitchell[1,2]假设，线粒体嵴膜、叶绿体颗粒膜和细菌质膜的ATP酶系统通过将ATP的可逆水解与质子跨膜的易位耦合，发挥重要的生物能量功能。随后报道了在ATP水解过程中，通过适当的pH值直接观察到线粒体[3-5]~叶绿体[6]和光合细菌[7]的染色质的质子移位。然而，尽管Harold[8]的重要和有启发性的工作，非光合细菌的atp酶的质子转移特性似乎还没有被直接证明。在缺乏这一关键信息的情况下，研究细菌atp酶系统的学生不幸地倾向于将这些系统中易于分离的水溶性片段（对应于线粒体和叶绿体atp酶[9]的成分F）视为“纯atp酶”或简称为“atp酶”。本文试图通过直接的pH测量表明，在大肠杆菌制备的膜囊泡对ATP的dccd敏感水解过程中，质子发生了移位，从而鼓励对细菌ATP酶系统进行更多功能相关的研究。*目前地址：剑桥大学生物化学系，网球场路，剑桥CB2 IQW， UK缩写：DCCD：双环己基碳酰亚胺；FCCP：羰基氰化物对三氟甲氧基苯腙；ph - 10gro（外水相中H+离子的化学活性）；-+ H+/P：质子易位商，表示水解每个ATP分子所易位的质子数。
It was postulated by Mitchell [1, 2] that the ATPase systems of mitochondrial cristae membranes, chloroplast grana membranes and bacterial plasma membranes perform an essential bioenergetic function by coupling the reversible hydrolysis of ATP to the translocation of protons across the membrane. Direct observation of proton translocation by appropriate pH measurements during ATP hydrolysis was subsequently reported for mitochondria [3-5]~ chloroplast grana [6] and chromatophores from photosynthetic bacteria [7]. However, in spite of the important and suggestive work of Harold [8], the proton-translocating property of the ATPases of non-photosynthetic bacteria does not yet seem to have been directly demonstrated. In the absence of this crucial information, students of bacterial ATPase systems have unfortunately tended to regard an easily-dissociable water-soluble fragment of these systems (corresponding to the F, component of mitochondrial and chloroplast ATPases [9]) as the ‘pure ATPase’or simply as ‘the ATPase’.. The present paper attempts to encourage more functionally relevant studies of bacterial ATPase systems by showing, with direct pH measurements, that protons are translocated during the DCCD-sensitive hydrolysis of ATP by membrane vesicles prepared from Escherichia coli.* Present address: Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 IQW, UK Abbreviations: DCCD: dicyclohexylcarbodi-imide; FCCP: carbonylcyanide p-trifluoromethoxyphenylhydrazone; pHo-1ogro (chemical activity of H+ ions in the outer aqueous phase);-+ H+/P: proton translocation quotient giving number of protons translocated per ATP molecule hydrolysed.