Diverse application platform for hard X-ray diffraction in SACLA (DAPHNIS): application to serial protein crystallography using an X-ray free-electron laser.
Diverse application platform for hard X-ray diffraction in SACLA (DAPHNIS): application to serial protein crystallography using an X-ray free-electron laser.
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DOI：
10.1107/s1600577515004464
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发表时间：
2015-05
影响因子：
2.5
通讯作者：
Yabashi M
中科院分区：
文献类型：
--
作者：
Tono K;Nango E;Sugahara M;Song C;Park J;Tanaka T;Tanaka R;Joti Y;Kameshima T;Ono S;Hatsui T;Mizohata E;Suzuki M;Shimamura T;Tanaka Y;Iwata S;Yabashi M
介绍了用x射线自由电子激光器进行连续飞秒晶体学的实验平台及其在SACLA的应用。利用x射线自由电子激光器（XFEL）研制了一套连续飞秒晶体学实验系统。它基本上由一个样品室、流体注射器和一个二维探测器组成。腔室和喷射器在1atm的氦气气氛下工作。环境压力操作有利于流体样品的应用。采用三种不同的注入器对水溶液或高粘性流体中的随机取向晶体进行注入。利用SPring-8埃紧凑自由电子激光器（SACLA）的10 keV XFEL对溶菌酶晶体进行了实验。在分辨率为2.4 Å的1µm晶体上获得了模型蛋白溶菌酶的结构。
An experimental platform for serial femtosecond crystallography using an X-ray free-electron laser and its applications at SACLA are described. An experimental system for serial femtosecond crystallography using an X-ray free-electron laser (XFEL) has been developed. It basically consists of a sample chamber, fluid injectors and a two-dimensional detector. The chamber and the injectors are operated under helium atmosphere at 1 atm. The ambient pressure operation facilitates applications to fluid samples. Three kinds of injectors are employed to feed randomly oriented crystals in aqueous solution or highly viscous fluid. Experiments on lysozyme crystals were performed by using the 10 keV XFEL of the SPring-8 Angstrom Compact free-electron LAser (SACLA). The structure of model protein lysozyme from 1 µm crystals at a resolution of 2.4 Å was obtained.