Genetic Characterization of 13 Subtype CRF01_AE Near Full-Length Genomes in Guangxi, China
Genetic Characterization of 13 Subtype CRF01_AE Near Full-Length Genomes in Guangxi, China
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中国广西13个亚型CRF01_AE近全长基因组的遗传特征
DOI：
10.1089/aid.2010.0026
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发表时间：
2010-06-01
影响因子：
1.5
通讯作者：
Li, Jingyun
中科院分区：
文献类型：
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作者：
Li, Lin;Liang, Shujia;Li, Jingyun
广西是华南地区艾滋病传播的重要中转地。该地区流行的HIV-1全长基因组的特征对于系统发育分析和疫苗开发非常重要。CRF01_AE是广西流行最快的亚型之一。在这项研究中，我们报告了13个近全长CRF01_AE基因组来自中国广西。将几乎全长的基因组逆转录并扩增成具有1-kb重叠区域的两半。PCR产物直接测序。序列分析表明，13株菌株均为CRF01_AE重组亚型。所有序列与越南和中国福建的序列形成两个聚类，表明CRF01_AE菌株在广西有多次传入。本研究结果将有助于进一步了解我国南方地区CRF01_AE毒株的系统发育关系，并为研制成功的HIV疫苗提供参考。
Guangxi is an important transit area for HIV transmission in South China. Characterization of the full-length genome of HIV-1 prevalent in the area is important for phylogenetic analysis and vaccine development. CRF01_AE is one of the most rapidly spreading subtypes in Guangxi. In this study, we reported thirteen near full-length CRF01_AE genomes from Guangxi, China. The nearly full-length genome was reverse transcripted and amplified in two halves with the 1-kb overlap regions. The PCR products were sequenced directly. The sequence analysis showed that all of 13 strains were CRF01_AE recombinant subtypes. Two clusters were set up with all of the sequences that grouped separately with sequences from Vietnam and Fujian, China, which strongly suggested multiple introductions of CRF01_AE strains into Guangxi province. The results will improve our understanding on the phylogenetic relationship of CRF01_AE strains in South China and also help in the development of a successful HIV vaccine.