RalA interacts with ZONAB in a cell density-dependent manner and regulates its transcriptional activity
RalA interacts with ZONAB in a cell density-dependent manner and regulates its transcriptional activity
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DOI：
10.1038/sj.emboj.7600497
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发表时间：
2005-01-12
期刊：
影响因子：
11.4
通讯作者：
Marshall, CJ
中科院分区：
文献类型：
--
作者：
Frankel, P;Aronheim, A;Marshall, CJ
Ral 蛋白是小 GTP 酶 Ras 超家族的成员，参与肌动蛋白细胞骨架重塑、细胞周期控制、细胞转化和囊泡运输的信号通路。为了鉴定新型 RalA 效应蛋白，我们使用反向 Ras 募集系统，发现 RalA 以 GTP 依赖性方式与 Y-box 转录因子 ZO-1 相关核酸结合蛋白 (ZONAB) 相互作用。随着上皮细胞变得更加致密并且细胞接触增加，RalA-ZONAB复合物的量增加。 RalA - ZONAB 相互作用导致 ZONAB 调节的启动子的转录抑制减轻。此外，致癌 Ras 的表达以 RalA 依赖性方式减轻了 ZONAB 的转录抑制。这里提供的数据表明 RalA/ZONAB 相互作用在 ZONAB 功能的调节中。
Ral proteins are members of the Ras superfamily of small GTPases and are involved in signalling pathways for actin cytoskeleton remodelling, cell cycle control, cellular transformation and vesicle transport. To identify novel RalA effector proteins, we used the reverse Ras recruitment system and found that RalA interacts with a Y- box transcription factor, ZO- 1- associated nucleic acid- binding protein ( ZONAB), in a GTP- dependent manner. The amount of the RalA - ZONAB complex increases as epithelial cells become more dense and increase cell contacts. The RalA - ZONAB interaction results in a relief of transcriptional repression of a ZONAB- regulated promoter. Additionally, expression of oncogenic Ras alleviates transcriptional repression by ZONAB in a RalA- dependent manner. The data presented here implicate the RalA/ ZONAB interaction in the regulation of ZONAB function.