Improvement of myocardial glycolipid metabolic disorder in diabetic hamster with Astragalus polysaccharides treatment
Improvement of myocardial glycolipid metabolic disorder in diabetic hamster with Astragalus polysaccharides treatment
复制标题
黄芪多糖治疗改善糖尿病仓鼠心肌糖脂代谢紊乱
DOI：
10.1007/s11033-012-1595-y
复制
发表时间：
2012-07-01
影响因子：
2.8
通讯作者：
Ye, Hong-Ying
中科院分区：
文献类型：
--
作者：
Chen, Wei;Xia, Yan-Ping;Ye, Hong-Ying
本研究的目的是观察黄芪多糖（APS）对糖尿病（DM）仓鼠心肌糖脂代谢的影响，探讨其干预DM心肌病的机制。低剂量链脲佐菌素诱导的仓鼠（STZ，40 mg/kg x 3 天，腹腔注射）血糖> 13.9 mmo/L 被视为 2 型糖尿病模型。测定正常对照组、DM组和APS治疗组仓鼠的血糖、血脂、胰岛素、C肽、心肌酶水平、心肌糖原染色、心肌超微结构、荧光定量RT-PCR检测心肌PPAR-α及其靶基因(FATP、ACS)和GLUT4 mRNA表达量。 DM组较正常组存在明显的糖脂代谢紊乱。 APS治疗组血糖、糖化血清蛋白、心肌酶和血脂水平较DM组显着降低，但胰岛素和C肽水平无差异。 APS治疗组心肌糖原染色及异常心肌超微结构较DM组明显改善。 APS组心肌PPAR-α及其靶基因(FATP、ACS)基因表达量显着低于DM组,而APS组GLUT4基因表达量高于DM组。 APS可部分改善糖尿病仓鼠心肌糖脂代谢紊乱，对心肌有一定保护作用。
The objective of the present study is to observe the effect of Astragalus polysaccharide (APS) on myocardial glucose and lipid metabolism in diabetes (DM) hamster and to explore its mechanism in intervention of DM cardiomyopathy. Low-dose- streptozotocin-induced hamsters (STZ, 40 mg/kg x 3 days, i.p.) with blood glucose > 13.9 mmo/L were considered as type 2 diabetic models. We measure blood glucose, serum lipid, insulin, C-peptide, myocardial enzyme levels, myocardial glycogen staining, myocardial ultrastructure, fluorescence quantitative RT-PCR detection of myocardial PPAR-alpha and the target genes (FATP, ACS) and GLUT4 mRNA expression in normal control group, DM group and APS treatment group hamsters. There was significant glycolipid metabolic disorders in DM group compared with normal group. Glucose, glycosylated serum protein, myocardial enzymes and lipid levels in APS treatment group decreased significantly than DM group, but insulin and C-peptide levels was no difference. Myocardial glycogen staining and abnormal myocardial ultrastructure in APS treatment group were significantly improved than in DM group. Gene expression of myocardial PPAR-alpha and its target genes (FATP, ACS) in APS group were significantly lower than in DM group, while gene expression of GLUT4 in APS group was higher than DM group. APS can partially improve myocardial glucose and lipid metabolism disorders in diabetic hamsters and protect myocardium in some extent.