STUDIES ON ENZYME VARIATION IN MURINE MALARIA PARASITES PLASMODIUM-BERGHEI, P-YOELII, PLASMODIUM-VINCKEI AND PLASMODIUM-CHABAUDI BY STARCH-GEL ELECTROPHORESIS
STUDIES ON ENZYME VARIATION IN MURINE MALARIA PARASITES PLASMODIUM-BERGHEI, P-YOELII, PLASMODIUM-VINCKEI AND PLASMODIUM-CHABAUDI BY STARCH-GEL ELECTROPHORESIS
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DOI：
10.1017/s0031182000048137
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发表时间：
1978-01-01
期刊：
影响因子：
2.4
通讯作者：
CARTER, R
中科院分区：
文献类型：
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作者：
CARTER, R
本文研究了非洲小鼠疟原虫伯氏疟原虫、约氏疟原虫、文氏疟原虫和查鲍迪疟原虫及其亚种的葡萄糖磷酸异构酶、6-磷酸葡萄糖脱氢酶、乳酸脱氢酶和谷氨酸脱氢酶(依赖于NADP)的电泳变异。采用水平淀粉凝胶电泳法。在每个亚种中检测到的分离物数量从1个(黑杨)到24个(查鲍迪)不等。大多数亚种的菌株之间存在着广泛的酶变异，可供研究的菌株超过2个。酶的多态现象在疟疾寄生虫中很常见。除了伯氏假单胞菌和约氏假单胞菌具有相同的乳酸脱氢酶的电泳型外，这4个种之间没有任何一种酶的同源性。小鼠疟原虫。在每个物种中，每个亚种之间共享共同的酶形式。亚种之间的区别在于至少有一种酶的电泳形式。酶在单一亚种分离株中的分布和重新分类符合疟疾寄生虫作为有性繁殖生物体的概念。对野生啮齿动物个体宿主中存在的寄生虫之间的差异的研究表明，自然疟疾感染通常包括遗传异质性的寄生虫种群。然而，单个受感染宿主中存在的任何一种寄生虫在遗传上截然不同的类型的数量似乎很少。通常，在一只受感染的动物中，存在不超过2到3个具有不同遗传结构的寄生虫克隆。
Electrophoretic variation of the enzymes glucose phosphate isomerase, 6-phosphogluconate dehydrogenase, lactate dehydrogenase and glutamate dehydrogenase (NADP-dependent) was studied in the African murine malaria parasites P. berghei, P. yoelii, P. vinckei and P. chabaudi and their subspecies. Horizontal starch gel electrophoresis was used. The number of isolates examined in each subspecies varied from 1 (P. y. nigeriensis) to 24 (P. c. chabaudi). Extensive enzyme variation was found among isolates of most of the subspecies from which more than 2 isolates were available for study. Enzyme polymorphism is of common occurrence among malaria parasites. With the exception of P. berghei and P. yoelii, of which all isolates share an identical electrophoretic form of lactate dehydrogenase, no enzyme forms are shared between any of the 4 spp. of murine plasmodia. Within each species common enzyme forms are shared among each of the subspecies. The subspecies are distinguished from each other by the electrophoretic forms of at least 1 enzyme. The distribution and reassortment of enzyme variation among isolates of a single subspecies is in accordance with the concept of malaria parasites as sexually reproducing organisms. The study of variation among parasites present in individual wild-caught rodent hosts demonstrates that natural malarial infections usually comprise genetically heterogeneous populations of parasites. Nevertheless, the number of genetically distinct types of parasite of any one species present in a single infected host appears small. Generally not more than 2 or 3 clones of parasite of distinct genetic constitution are present in a single infected animal.