Mechanical impact of epithelial-mesenchymal transition on epithelial morphogenesis in Drosophila
Mechanical impact of epithelial-mesenchymal transition on epithelial morphogenesis in Drosophila
复制标题
DOI：
10.1038/s41467-019-10720-0
复制
发表时间：
2019-07-04
影响因子：
16.6
通讯作者：
Suzanne, Magali
中科院分区：
文献类型：
--
作者：
Gracia, Melanie;Theis, Sophie;Suzanne, Magali
上皮-间质转化（EMT）是一个重要的生理和病理过程。有趣的是，EMT通常与发育过程中的组织内陷有关;然而，EMT对组织重塑的影响仍未被探索。在这里，我们表明，在开始的EMT过程中，细胞产生的apico-basal力，正交的上皮细胞的表面，构成了一个重要的驱动力，果蝇组织内陷。当异位诱导EMT时，开始脱层的细胞产生正交力并诱导异位折叠。类似地，在中胚层内陷过程中，经历EMT的细胞通过形成肌球蛋白II的顶基结构产生顶基力。使用激光显微切割和计算机物理建模，我们表明，中胚层内陷不进行，如果apico-basal力受损，这表明它们构成的驱动力在折叠过程中。总之，这些数据揭示了EMT对形态发生的机械影响。
Epithelial-mesenchymal transition (EMT) is an essential process both in physiological and pathological contexts. Intriguingly, EMT is often associated with tissue invagination during development; however, the impact of EMT on tissue remodeling remain unexplored. Here, we show that at the initiation of the EMT process, cells produce an apico-basal force, orthogonal to the surface of the epithelium, that constitutes an important driving force for tissue invagination in Drosophila. When EMT is ectopically induced, cells starting their delamination generate an orthogonal force and induce ectopic folding. Similarly, during mesoderm invagination, cells undergoing EMT generate an apico-basal force through the formation of apicobasal structures of myosin II. Using both laser microdissection and in silico physical modelling, we show that mesoderm invagination does not proceed if apico-basal forces are impaired, indicating that they constitute driving forces in the folding process. Altogether, these data reveal the mechanical impact of EMT on morphogenesis.