STUDIES ON THE PROTEINS OF MASS-CULTIVATED, BLUE-GREEN-ALGA (SPIRULINA-PLATENSIS)
STUDIES ON THE PROTEINS OF MASS-CULTIVATED, BLUE-GREEN-ALGA (SPIRULINA-PLATENSIS)
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DOI：
10.1021/jf00105a022
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发表时间：
1981-01-01
影响因子：
6.1
通讯作者：
VENKATARAMAN, LV
中科院分区：
文献类型：
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作者：
DEVI, MA;SUBBULAKSHMI, G;VENKATARAMAN, LV
研究了淡水、批量培养的白颡鱼的蛋白质特性。测定了该藻蛋白在水和各种水溶液中的溶解度。蓝绿藻的总蛋白质含量约为。50-55%，其中近9.9%为非蛋白氮。用水连续提取3次，可提取总蛋白氮的80%左右。该藻蛋白的等电点为3.0。用标准技术对总蛋白进行了理化表征。在超离心条件下，总蛋白分解为s20、w[沉降系数]分别为2.6和4.7 s的2个主要组分，总蛋白的聚丙烯酰胺电泳图谱显示出7个条带，其中3个条带较为突出。用胃蛋白酶-胰酶系统测定时，发现新鲜藻类总蛋白的体外消化率为85%。
The characteristics of the protein of fresh-water, mass-cultured S. platensis were studied. The solubility of this algal protein in water and various aqueous solvents was estimated. The total protein content of the blue-green algae was .apprx. 50-55% of which nearly 9.9% was nonprotein nitrogen. About 80% of the total protein nitrogen can be extracted by 3 successive extractions with water. The isoelectric point of this algal protein is 3.0. The total proteins were characterized physicochemically by standard techniques. In the ultracentrifuge total proteins resolve into 2 major components with s20,w [sedimentation coefficient] values of 2.6 and 4.7 S. The polyacrylamide gel electrophoretic pattern of the total protein showed 7 bands including 3 prominent ones. The in vitro digestibility of the total protein of fresh algae was found to be 85% when assayed with a pepsin-pancreatin system.