PARACRYSTALLINE SHEETS REAGGREGATED FROM SOLUBILIZED EXOSPORIUM OF BACILLUS-CEREUS
PARACRYSTALLINE SHEETS REAGGREGATED FROM SOLUBILIZED EXOSPORIUM OF BACILLUS-CEREUS
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DOI：
10.1128/jb.107.1.320-324.1971
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发表时间：
1971-01-01
影响因子：
3.2
通讯作者：
GERHARDT, P
中科院分区：
文献类型：
--
作者：
BEAMAN, TC;PANKRATZ, HS;GERHARDT, P
用十二烷基硫酸钠（SDS）或苯酚和乙酸处理从蜡状芽孢杆菌（Bacillus cereus）休眠孢子中分离的孢子外壁碎片。离心各制备物后，通过圆盘凝胶电泳将上清液中的蛋白质分别分离成两条或八条带。SDS-溶解的级分含有直径为11至44 nm的球形颗粒。当离心直至澄清时，透析后的该部分仍然产生晶体状片，其具有与天然外孢壁的片段相同的晶格对称性和主要化学成分（蛋白质、脂质和碳水化合物）。
Fragments of exosporium, isolated from dormant spores ofBacillus cereus, were disintegrated by treatment with sodium dodecyl sulfate (SDS) or with phenol and acetic acid. After centrifugation of each preparation, proteins in the supernatant fractions were resolved by disc gel electrophoresis into either two or eight bands, respectively. The SDS-solubilized fraction contained spheroidal particles 11 to 44 nm in diameter. When centrifuged until clear, this fraction after dialysis still gave rise to crystal-like sheets which had the same lattice symmetry and major chemical components (protein, lipid, and carbohydrate) as fragments of the native exosporium.