Reconstruction of a vascular bed with perfusable blood vessels using a decellularized porcine small intestine for clinical application.
Reconstruction of a vascular bed with perfusable blood vessels using a decellularized porcine small intestine for clinical application.
复制标题
利用脱细胞猪小肠重建可灌注血管的血管床用于临床应用。
DOI：
10.1007/978-3-030-66169-4_35
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Mitsuo Umezu.
中科院分区：
文献类型：
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作者：
Yusuke Tobe;Katsuhisa Sakaguchi;Jun Homma;Kazunori Sano;Eiji Kobayashi;Hidekazu Sekine;Kiyotaka Iwasaki;Tatsuya Shimizu;Mitsuo Umezu.
工程化的三维组织在体外再生治疗是非常需要的。动脉和静脉可连接的血管床在体外构建三维组织和提高体内移植三维组织的存活率方面起着至关重要的作用。在这里，我们开发了一种方法来重建一种新的血管床，可以植入人体用于临床应用。选择有动静脉环的猪小肠作为血管床骨架，用洗涤剂和酶进行血管内灌流脱细胞。随后，用人体细胞重新内皮化脱细胞的小肠移植物，使血液进入血管床。小肠移植物成功脱细胞，对移植物爆破压无严重损伤，大鼠移植2周内无严重炎症反应。此外，脱细胞的小肠移植物在7天内与人内皮细胞部分血运。因此，重建的肠血管床可作为一种具有临床研究潜力的人体可移植血管床。
Engineering three-dimensional tissuesin vitrofor regenerative therapy is highly desired. Vascular beds with connectable arteries and veins play crucial roles in fabricating three-dimensional tissuesin vitroand improving the survival rate of transplanted three-dimensional tissuesin vivo. Here, we developed a method to reconstruct a new vascular bed that could be implanted into humans for clinical applications. Porcine small intestine with an arteriovenous loop was selected as a vascular bed skeleton and decellularized using transvascular perfusion with both detergent and enzyme. Subsequently, the decellularized intestinal graft was re-endothelialized with human cells to allow blood perfusion into the vascular bed. The small intestinal graft was successfully decellularized without severe damage to the burst pressure of the graft, and the decellularized intestinal graft could be transplanted without a severe inflammatory response in rats for two weeks. In addition, the decellularized intestinal graft was partially vascularized with human endothelial cells in seven days. Therefore, the reconstructed intestinal vascular bed may serve as a human transplantable vascular bed with potential in clinical investigations.