Isotonic but not hypertonic ethanol stimulates LHRH secretion from perifused rat median eminence.
Isotonic but not hypertonic ethanol stimulates LHRH secretion from perifused rat median eminence.
复制标题
等渗而非高渗乙醇刺激灌注大鼠正中隆起的 LHRH 分泌。
DOI：
10.1159/000126541
复制
发表时间：
1993
影响因子：
4.1
通讯作者：
Greer,SE
中科院分区：
文献类型：
--
作者：
Inukai,T;Wang,X;Greer,MA;Greer,SE
我们利用促黄体生成激素释放激素（LHRH）分泌灌流切碎的大鼠下丘脑正中隆起（ME）组织，以评估是否等渗乙醇会刺激神经分泌，因为它没有分泌垂体细胞。等渗乙醇诱导LHRH分泌的剂量依赖性爆发，在2-3分钟时达到最大值，10分钟时恢复到接近基线。在Ca 2+耗尽的培养基（<2 µ MCa 2+）中，等渗乙醇对分泌的刺激增强，但去极化30 mM K+诱导的分泌被消除。高渗乙醇是无效的刺激LHRH分泌在正常或无钙培养基。下丘脑LHRH分泌细胞对乙醇的分泌反应及其由介质Ca 2+的负调节因此与正常垂体前叶细胞的相同，并且推测是由渗透性乙醇分子穿过质膜流入而引起的细胞肿胀引起的。
We utilized luteinizing hormone-releasing hormone (LHRH) secretion by perifused minced rat hypothalamic median eminence (ME) tissue to evaluate whether isotonic ethanol would stimulate neurosecretion as it does secretion from pituitary cells. Isotonic ethanol induced a dose-dependent burst of LHRH secretion which was maximal at 2-3 min and returned to near baseline by 10 min. In Ca2+-depleted media (<2 µMCa2+), stimulation of secretion by isotonic ethanol was enhanced, but secretion induced by depolarizing 30 mM K+was abolished. Hypertonic ethanol was ineffective in stimulating LHRH secretion in either normal or Ca2+-free media. The secretory response of hypothalamic LHRH-secreting cells to ethanol and its negative modulation by medium Ca2+is thus identical to that of normal anterior pituitary cells and presumably is caused by cell swelling resulting from influx of permeant ethanol molecules across the plasmalemma.