Prolactin-like protein-A is a functional modulator of natural killer cells at the maternal-fetal interface
Prolactin-like protein-A is a functional modulator of natural killer cells at the maternal-fetal interface
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DOI：
10.1016/s0303-7207(03)00125-4
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发表时间：
2003-06-30
影响因子：
4.1
通讯作者：
Soares, MJ
中科院分区：
文献类型：
--
作者：
Ain, R;Tash, JS;Soares, MJ
自然杀伤（NK）细胞是主要淋巴细胞存在于健康的啮齿动物和人类植入部位。在大鼠中，NK细胞从发育中的绒毛膜尿囊性胎盘的扩张、分化和随后的迁移与一种新的妊娠和滋养细胞特异性细胞因子——催乳素（PRL）样蛋白a （PLP-A）的表达一致。PLP-A特异性结合子宫NK细胞，但似乎不利用PRL的受体系统。在本报告中，我们发现PLP-A与NK细胞的相互作用不是由已知NK细胞功能调节剂（包括白细胞介素-2）所利用的受体介导的。白细胞介素-7，白细胞介素-12和白细胞介素-15 （IL-15）。子宫NK细胞对PLP-A或IL-15的反应是细胞内钙动员的增加。相反，与IL-15不同，PLP-A有效抑制NK细胞产生干扰素γ (IFNgamma)的能力，IFNgamma是NK细胞功能的关键介质。胎盘PLP-A的表达与蜕膜中ifnγ的表达成反比。胎盘中PLP-A表达的增加与胎盘附近蜕膜中IFNgamma含量的下降一致。综上所述，滋养层细胞来源的PLP-A有助于调节母胎界面的NK细胞，以确保胚胎的正常生长发育。(C) 2003爱思唯尔科学爱尔兰有限公司版权所有。
Natural killer (NK) cells are the predominant lymphocytes present in healthy rodent and human implantation sites. In the rat, the expansion, differentiation and subsequent migration of NK cells away from the developing chorioallantoic placenta coincide with the expression of a novel pregnancy- and trophoblast cell-specific cytokine, prolactin (PRL)-like protein A (PLP-A). PLP-A specifically binds to uterine NK cells but does not appear to utilize receptor systems for PRL. In the present report, we show that PLP-A interactions with NK cells are not mediated by receptors utilized by known modulators of NK cell function, including interleukin-2. interleukin-7, interleukin-12, and interleukin-15 (IL-15). Uterine NK cells respond to PLP-A or IL-15 with an increase in intracellular calcium mobilization. In contrast, PLP-A, unlike IL-15, effectively suppresses the ability of NK cells to produce interferon-gamma (IFNgamma), a key mediator of NK cell function. Placental PLP-A expression is reciprocal to mesometrial decidua expression of IFNgamma. Increased expression of PLP-A by the placenta coincides with the decline of IFNgamma content in the mesometrial decidua adjacent to the placenta. In summary, trophoblast cell-derived PLP-A contributes to the regulation of NK cells at the maternal-fetal interface to ensure appropriate embryonic growth and development. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.