Upconversion fluorescence imaging of cells and small animals using lanthanide doped nanocrystals
Upconversion fluorescence imaging of cells and small animals using lanthanide doped nanocrystals
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DOI：
10.1016/j.biomaterials.2007.10.051
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发表时间：
2008-03-01
期刊：
影响因子：
14
通讯作者：
Zhang, Yong
中科院分区：
文献类型：
--
作者：
Chatteriee, Dev K.;Rufalhah, Abdul J.;Zhang, Yong
近红外区域激发的上转换荧光成像技术已被用于生物细胞和组织的成像。这种方法有几个优点，包括对生物体没有光损伤，非常低的自荧光，高检测灵敏度。，光在生物组织中的穿透深度高。在本报告中，我们展示了一种新的上转换荧光团，镧系掺杂纳米晶体，用于动物细胞和一些深层组织的成像。合成了聚乙烯亚胺（PEI）包被的NaYF4:Yb，Er纳米粒子，在近红外激光980 nm激发下产生很强的上转换荧光。纳米颗粒在生理缓冲盐水（PBS）中稳定，对骨髓stein细胞无毒，耐光漂白。在980 nm的近红外激光照射下，将纳米颗粒注入某些细胞系或皮下和肌肉内注射到一些靠近体表或身体深处的组织中，显示出可见的荧光。据我们所知，这是第一次使用上转换荧光团进行细胞和组织成像的演示。(c) 2007 Elsevier Ltd.版权所有。
Upconversion fluorescence imaging technique with excitation in the near-infrared (NIR) region has been used for imaging of biological cells and tissues. This has several advantages, including absence of photo-damage to living organisms, very low auto-fluorescence, high detection sensitivity., and high light penetration depth in biological tissues. In this report we demonstrate the use of a new upconversion fluorophore, lanthanide doped nanocrystals, for imaging of cells and some deep tissues in animal. Polyethyleneimine (PEI) coated NaYF4:Yb,Er nanoparticles were synthesized, which produce very strong Upconversion fluorescence when excited at 980 nm by a NIR laser. The nanoparticles were shown to be stable in physiologic buffered saline (PBS), non-toxic to bone marrow stein cells, and resistant to photo-bleaching. The nanoparticles delivered into some cell lines or injected intradermally and intramuscularly into some tissues either near the body surface or deep in the body of rats showed visible fluorescence, when exposed to a 980 nm NIR laser. To the best of our knowledge, this represents the first demonstration of use of upconversion fluorophores for cellular and tissue imaging. (c) 2007 Elsevier Ltd. All rights reserved.