Retention and paper-strength characteristics of anionic polyacrylamides conjugated with carbohydrate-binding modules
Retention and paper-strength characteristics of anionic polyacrylamides conjugated with carbohydrate-binding modules
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DOI：
10.15376/biores.4.1.234-244
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发表时间：
2008-12
期刊：
影响因子：
1.5
通讯作者：
S. Yokota;K. Matsuo;T. Kitaoka;H. Wariishi
中科院分区：
文献类型：
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作者：
S. Yokota;K. Matsuo;T. Kitaoka;H. Wariishi
研究了具有糖水解酶对纸浆纤维的特定亲和力的聚合物的保留行为，以增强受污染的造纸系统中的纸张强度。通过木瓜蛋白酶定点消化获得源自绿色木霉和里氏木霉的纤维素酶以及源自疏棉状嗜热丝孢菌的木聚糖酶的碳水化合物结合模块（CBM），然后通过肽缩合反应将其引入阴离子聚丙烯酰胺（A-PAM）中。三种类型的 CBM 共轭 A-PAM (CBM-A-PAM) 显示出不同的保留行为，具体取决于纸浆基材的类型，即硬木和软木纤维。来自 T. viride 的 CBM-A-PAM 对硬木纸浆纤维表现出良好的添加剂保留作用，即使在存在 Ca2+ 离子和木质素磺酸盐的污染条件下，纸张也具有高拉伸强度。来自里氏木霉的 CBM-A-PAM 对于软木板材表现出比硬木板材更好的性能。木聚糖酶 CBM-A-PAM 优先保留在可能存在半纤维素的硬木纤维上。这种添加剂保留系统具有酶对纸浆纤维的固有亲和力，有望扩大煤层气聚合物在实际湿部工艺中的应用范围。
The retention behavior of polymers having the specific affinities of glyco-hydrolases for pulp fibers was investigated with regard to paper-strength enhancement in contaminated papermaking systems. Carbohydrate-binding modules (CBMs) of cellulases derived from Trichoderma viride and T. reesei, and of xylanase from Thermomyces lanuginosus, were obtained by site-directed digestion with papain, then introduced into anionic polyacrylamide (A-PAM) via a peptide condensation reaction. Three types of CBM-conjugated A-PAMs (CBM-A-PAMs) displayed different retention behavior, depending on the kind of pulp substrates, i.e. hardwood and softwood fibers. The CBM-A-PAM from T. viride demonstrated good additive retention for hardwood pulp fibers, resulting in high tensile strength of paper sheets, even under contaminated conditions in the presence of Ca2+ ions and ligninsulfonate. The CBM-A-PAM from T. reesei showed better performance for softwood than for hardwood sheets. The xylanase CBM-A-PAM was preferentially retained on hardwood fibers in which hemicelluloses might be present. Such an additive retention system, with inherent affinities of enzymes for pulp fibers, is expected to expand the application range of CBM-polymers in practical wet-end processes.