Genetic diversity of Vibrio parahaemolyticus strains isolated from farmed Pacific white shrimp and ambient pond water affected by acute hepatopancreatic necrosis disease outbreak in Thailand
Genetic diversity of Vibrio parahaemolyticus strains isolated from farmed Pacific white shrimp and ambient pond water affected by acute hepatopancreatic necrosis disease outbreak in Thailand
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DOI：
10.1093/femsle/fnv222
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发表时间：
2016-01-01
影响因子：
2.1
通讯作者：
Iida, Tetsuya
中科院分区：
文献类型：
--
作者：
Chonsin, Kaknokrat;Matsuda, Shigeaki;Iida, Tetsuya
急性肝胰腺坏死病（AHPND）是一种引起养殖对虾大量死亡的新兴疾病。近年来亚洲爆发的AHPND给对虾养殖业造成了巨大损失，并已成为严重的社会经济问题。AHPND的病原体是副溶血性弧菌，通常已知其可引起人类食源性胃肠炎。然而，目前关于副溶血性弧菌AHPND菌株的流行病学研究报道较少，且AHPND菌株之间的遗传关系尚不清楚。在这里，我们报告了从泰国AHPND疫情中分离的副溶血性弧菌菌株的遗传特征。我们发现八个菌株AHPND疑似虾和池塘水是阳性的AHPND标记AP 1和AP 2。PCR分析证实，这8株AP阳性AHPND均不具有影响人类的常规毒力因子如耐热直接溶血素（TDH）、TDH相关溶血素（TRH）和III型分泌系统2的基因。多位点序列分型的系统发育分析表明，AHPND菌株的遗传多样性，这表明AHPND菌株不是来自一个单一的遗传谱系。我们的研究代表了使用多位点序列分型的AHPND引起的副溶血性弧菌菌株的分子流行病学的第一份报告，并提供了对其进化机制的深入了解。
Acute hepatopancreatic necrosis disease (AHPND) is an emerging shrimp disease that causes massive die-offs in farmed shrimps. Recent outbreaks of AHPND in Asia have been causing great losses for shrimp culture and have become a serious socioeconomic problem. The causative agent of AHPND is Vibrio parahaemolyticus, which is typically known to cause food-borne gastroenteritis in humans. However, there have been few reports of the epidemiology of V. parahaemolyticus AHPND strains, and the genetic relationship among AHPND strains is unclear. Here, we report the genetic characterization of V. parahaemolyticus strains isolated from AHPND outbreaks in Thailand. We found eight isolates from AHPND-suspected shrimps and pond water that were positive for AHPND markers AP1 and AP2. PCR analysis confirmed that none of these eight AP-positive AHPND strains possesses the genes for the conventional virulence factors affecting to humans, such as thermostable direct hemolysin (TDH), TDH-related hemolysin (TRH) and type III secretion system 2. Phylogenetic analysis by multilocus sequence typing showed that the AHPND strains are genetically diverse, suggesting that AHPND strains were not derived from a single genetic lineage. Our study represents the first report of molecular epidemiology of AHPND-causing V. parahaemolyticus strains using multilocus sequence typing, and provides an insight into their evolutionary mechanisms.