Mac-1low early myeloid cells in the bone marrow-derived SP fraction migrate into injured skeletal muscle and participate in muscle regeneration
Mac-1low early myeloid cells in the bone marrow-derived SP fraction migrate into injured skeletal muscle and participate in muscle regeneration
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DOI：
10.1016/j.bbrc.2004.07.069
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发表时间：
2004-09-03
影响因子：
3.1
通讯作者：
Takeda, S
中科院分区：
文献类型：
--
作者：
Ojima, K;Uezumi, A;Takeda, S
最近的研究表明，骨髓（BM）细胞，包括富含造血干细胞（HSC）的BM侧群（BM-SP）细胞，在再生过程中被整合到骨骼肌中，但尚不清楚BM细胞如何以及何种类型有助于肌纤维再生。我们发现，骨髓移植小鼠受伤后，大量 SP 细胞从骨髓迁移到肌肉。再生肌肉中这些源自骨髓的 SP 细胞表达与 HSC 不同的表面标记，并且无法重建小鼠血液系统。损伤后再生肌肉中骨髓衍生的 SP/Mac-1（低）细胞数量增加。重要的是，我们与活化卫星细胞的共培养研究表明，该部分具有显着的肌原分化潜力。相比之下，成熟的炎症细胞（Mac-1（高））显示出可忽略不计的生肌活性。此外，这些BM衍生的SP/Mac-1（低）细胞产生单核肌细胞，表明它们的肌生成不是由与宿主肌原细胞随机融合引起的，尽管它们需要与肌原细胞进行细胞间接触以进行肌肉分化。综上所述，我们的数据表明，HSC 和成熟炎症细胞，但 BM 来源的 SP 部分中的 Mac-1（低）早期骨髓细胞，在骨骼肌再生中发挥着重要作用。 (C) 2004 Elsevier Inc. 保留所有权利。
Recent studies have shown that bone marrow (BM) cells, including the BM side population (BM-SP) cells that enrich hematopoietic stem cells (HSCs), are incorporated into skeletal muscle during regeneration, but it is not clear how and what kinds of BM cells contribute to muscle fiber regeneration. We found that a large number of SP cells migrated from BM to muscles following injury in BM-transplanted mice. These BM-derived SP cells in regenerating muscles expressed different surface markers from those of HSCs and could not reconstitute the mouse blood system. BM-derived SP/Mac-1(low) cells increased in number in regenerating muscles following injury. Importantly, our co-culture studies with activated satellite cells revealed that this fraction carried significant potential for myogenic differentiation. By contrast, mature inflammatory (Mac-1(high)) cells showed negligible myogenic activities. Further, these BM-derived SP/Mac-1(low) cells gave rise to mononucleate myocytes, indicating that their myogenesis was not caused by stochastic fusion with host myogenic cells, although they required cell-to-cell contact with myogenic cells for muscle differentiation. Taken together, our data suggest that neither HSCs nor mature inflammatory cells, but Mac-1(low) early myeloid cells in the BM-derived SP fraction, play an important role in regenerating skeletal muscles. (C) 2004 Elsevier Inc. All rights reserved.