Characterization of a novel sRNA contributing to biofilm formation in Salmonella enterica serovar Typhimurium.
Characterization of a novel sRNA contributing to biofilm formation in Salmonella enterica serovar Typhimurium.
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DOI：
10.17912/micropub.biology.000796
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发表时间：
2023
影响因子：
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通讯作者：
Borchert, Glen M
中科院分区：
文献类型：
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作者：
Naaz, Sayema;Sakib, Najmuj;Houserova, Dominika;Badve, Rani;Crucello, Aline;Borchert, Glen M
小RNA(SRNAs)是一种约50-200个核苷酸的非编码短小RNA，被认为在细胞应激期间主要调节细菌的关键活动。本研究检测了营养缺乏的肠炎沙门氏菌血清型鼠伤寒沙门氏菌中特定的sRNA与生物膜形成的相关性。主要根据它们的基因组位置和/或假定的靶标，选择了8个独特的sRNA进行删除。定量和定性分析证实了其中一个，sRNA1186573，是肠球菌有效生物被膜形成所必需的，进一步强调了sRNAs在沙门氏菌应激反应中的重要性。
Small RNAs (sRNAs) are short noncoding RNAs of ~50-200 nucleotides believed to primarily function in regulating crucial activities in bacteria during periods of cellular stress. This study examined the relevance of specific sRNAs on biofilm formation in nutrient starved Salmonella enterica serovar Typhimurium. Eight unique sRNAs were selected for deletion primarily based on their genomic location and/or putative targets. Quantitative and qualitative analyses confirm one of these, sRNA1186573, is required for efficient biofilm formation in S. enterica further highlighting the significance of sRNAs during Salmonella stress response.