Improvement on in vitro maturation, fertilization and development of minke whale (Balaenoptera acutorostrata) oocytes
Improvement on in vitro maturation, fertilization and development of minke whale (Balaenoptera acutorostrata) oocytes
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DOI：
10.1016/s0093-691x(01)00585-4
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发表时间：
2001-09-01
期刊：
影响因子：
2.8
通讯作者：
Fukui, Y
中科院分区：
文献类型：
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作者：
Asada, M;Tetsuka, M;Fukui, Y
本研究旨在提高小须鲸卵母细胞的体外成熟、受精和后续发育水平。研究了不同浓度(0、10和20%)的胎鲸血清(FWS)对青春期前和成年小须鲸卵丘-卵母细胞复合体(COC)核成熟、形态分级(A、B级)的影响。在含20%FWS的培养液中，取自成年鲸鱼的A级(大于或等于5层卵丘细胞)COC含量较高(P
The aims of the present study were to improve in vitro maturation, fertilization and subsequent development of minke whale oocytes. We investigated the effects of different concentrations (0, 10 and 20%) of fetal whale serum (FWS) in maturation medium on nuclear maturation, morphological grade (A or B) of cumulus-oocyte complexes (COC) obtained from prepubertal and adult minke whales. Grade A (greater than or equal to5 layers of cumulus cells) COC collected from the adult whales and cultured in the medium with 20% FWS had a higher (P