Mild Electrical Stimulation Increases Ubiquitinated Proteins and Hsp72 in A549 Cells via Attenuation of Proteasomal Degradation
Mild Electrical Stimulation Increases Ubiquitinated Proteins and Hsp72 in A549 Cells via Attenuation of Proteasomal Degradation
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DOI：
10.1254/jphs.08180sc
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发表时间：
2008-10-01
影响因子：
3.5
通讯作者：
Kai, Hirofumi
中科院分区：
文献类型：
--
作者：
Morino, Saori;Suico, Mary Ann;Kai, Hirofumi
为了探讨温和电刺激（MES）的细胞效应，我们用5 V的低强度直流电处理A549细胞10 min。MES没有诱导细胞毒性或未折叠蛋白反应（UPR）。有趣的是，泛素化蛋白和热休克蛋白（Hsp）72的表达增加，而不是其他热休克蛋白。MES减弱了Hsp 72的降解，Hsp 72是蛋白酶体-泛素系统的底物。这些结果，沿着观察到的泛素化蛋白表达的增加，暗示MES可能影响调节许多蛋白命运的蛋白酶体系统。
To explore the cellular effects of mild electrical stimulation (MES), we treated A549 cells with low-intensity direct current at 5 V applied for 10 min. MES did not induce cell cytotoxicity or the unfolded protein response (UPR). Interestingly, the expression of ubiquitinated proteins and heat shock protein (Hsp) 72 was increased but not that of other Hsps. MES attenuated the degradation of Hsp72, which is a substrate of the proteasome-ubiquitin system. These results, along with the observed increase in expression of ubiquitinated proteins, imply that MES may affect the proteasome system, which regulates the fate of many proteins.