REGIONAL DIFFERENCES IN METHYL FARNESOATE PRODUCTION BY THE LOBSTER MANDIBULAR ORGAN
REGIONAL DIFFERENCES IN METHYL FARNESOATE PRODUCTION BY THE LOBSTER MANDIBULAR ORGAN
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DOI：
10.2307/1542032
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发表时间：
1994-02-01
影响因子：
1.6
通讯作者：
COUCH, EF
中科院分区：
文献类型：
--
作者：
BORST, DW;TSUKIMURA, B;COUCH, EF
从龙虾，Homarus americanus的下颌器官（MO）的视觉检查，揭示了两个不同的形态区域：一个扇形折叠区域沿着一个边缘的腺体，和一个光滑的，未折叠的区域，包括其余的腺体。因为MO产生法呢酸甲酯（MF），所以测量了两个区域的MF含量。在新鲜解剖的腺体中，超过95%的MF被发现在腺体的扇形折叠区域。在与[H-3-甲基]甲硫氨酸（MF的放射性标记前体）孵育的MO切片中，发现90%以上的MF合成位于扇形折叠区域。眼柄切除，一个程序，增加MO活动，造成MF含量的MO增加超过130倍，但对MF的区域分布影响不大。组织学观察表明，这两个区域有不同的细胞组成。扇形折叠区包含两种细胞类型（A和B）。A细胞有丝分裂活跃，似乎未分化。B细胞内有大量小空泡。未折叠区域主要由第三细胞类型（C）组成。C细胞体积大，形态复杂，含有许多线粒体和大的空泡样结构。它们含有相对较少的小液泡。根据外观和位置，B细胞似乎是MF合成的可能位点。C细胞的生理重要性尚不清楚。
Visual examination of the mandibular organ (MO) from the lobster, Homarus americanus, disclosed two distinct morphological regions: a fan-folded region along one edge of the gland, and a smooth, unfolded region comprising the rest of the gland. Because MOs produce methyl farnesoate (MF), the MF content of both regions was measured. In freshly dissected glands, more than 95% of the MF was found in the fan-folded region of the gland. In MO sections incubated with [H-3-methyl] methionine (a radiolabeled precursor of MF), more than 90% of MF synthesis was found in the fan-folded region. Eyestalk ablation, a procedure that increases MO activity, caused the MF content of MOs to increase more than 130-fold, but had little effect on the regional distribution of MF. Histological observations indicated that these two regions had different cellular compositions. The fan-folded region contained two cell types (A and B). The A cells were mitotically active and appeared to be undifferentiated. The B cells contained a large number of small vacuoles. The unfolded region was largely composed of a third cell type (C). The C cells were large and morphologically complex, containing many mitochondria and large vacuolar-like structures. They contained relatively few small vacuoles. On the basis of appearance and location, B cells appear to be the likely site of MF synthesis. The physiological importance of C cells is unknown.