Study on Solubilization and Stabilization of Eight Flavonoids by 17 Chinese Herbal Polysaccharides
Study on Solubilization and Stabilization of Eight Flavonoids by 17 Chinese Herbal Polysaccharides
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17种中药多糖增溶和稳定8种黄酮类化合物的研究
DOI：
10.1155/2020/6235284
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发表时间：
2020-08-26
影响因子：
3
通讯作者：
Deng, Yanru
中科院分区：
文献类型：
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作者：
Cao, Xuexiao;Wang, Lei;Deng, Yanru
黄酮类化合物具有多种生物活性，是中药的重要活性成分。采用高效液相色谱法（HPLC）研究了17种多糖与8种黄酮类化合物之间的相互作用及其对黄酮类化合物溶解性和稳定性的影响。通过统计方法分析了多糖对黄酮溶解度的影响，并显示出显着的溶解度改善。采用恒温加速法（90°C/pH 9缓冲溶液）测定了含多糖和不含多糖的黄酮类化合物的降解动力学和半衰期。所有的多糖都显示出对所有8种黄酮类化合物的稳定作用。其稳定作用顺序为：槲皮素、黄芩素>黄芩苷>木犀草素>大豆苷>芦丁>毛地黄黄酮>大豆苷。采用相溶解度法研究了槲皮素和黄芩素的作用机理。结果表明，两种黄酮类化合物均能与多糖形成1：1的包合物，这可能是提高其溶解性和稳定性的因素之一。这些发现增加了我们对中药中内源性多糖在提高生物活性黄酮的稳定性和生物利用度中的作用的理解。
Flavonoids are important active components of traditional Chinese medicines (TCMs) because of their many biological activities. We studied the interaction between 17 polysaccharides and eight flavonoids via high-performance liquid chromatography (HPLC) and the effect of the interactions on the solubility and stability of the flavonoids. The effect of the polysaccharides on the solubility of flavonoids was analyzed by statistical methods and showed significant solubility improvements. The constant temperature acceleration method (90°C/pH 9 buffer solution) was used to measure the degradation kinetics and half-life of flavonoids with and without polysaccharides. All the polysaccharides displayed a stabilizing effect on all eight flavonoids. The stabilizing effects varied in the order: quercetin, baicalein > baicalin > galuteolin > daidzin > rutin > luteolin > daidzien. The phase-solubility method was applied to quercetin and baicalein to study the mechanism of action of the polysaccharides. It appeared that the two flavonoids could form 1 : 1 inclusion complexes with polysaccharides, which may be one of the factors increasing solubility and stability. These findings increase our understanding of the role of endogenous polysaccharides in TCM in improving the stability and bioavailability of bioactive flavonoids.