SOX10-Cre-Labeled Cells Under the Tongue Epithelium Serve as Progenitors for Taste Bud Cells That Are Mainly Type III and Keratin 8-Low.
SOX10-Cre-Labeled Cells Under the Tongue Epithelium Serve as Progenitors for Taste Bud Cells That Are Mainly Type III and Keratin 8-Low.
复制标题
舌上皮下 SOX10-Cre 标记的细胞充当味蕾细胞的祖细胞，味蕾细胞主要为 III 型和角蛋白 8-Low。
DOI：
10.1089/scd.2020.0022
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发表时间：
2020
影响因子：
4
通讯作者：
Liu,Hong-Xiang
中科院分区：
文献类型：
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作者：
Yu,Wenxin;Ishan,Mohamed;Yao,Yao;Stice,StevenL;Liu,Hong-Xiang
味蕾细胞是经历连续更新的特化上皮细胞，因此需要活跃的祖细胞用于其更新和完整的味觉功能。我们之前的研究表明，味蕾细胞群起源于周围舌上皮细胞的外部，而舌上皮细胞以前被认为是味蕾祖细胞的唯一来源。在这项研究中，我们证明了SOX 10（SRY相关的HMG盒基因10）表达细胞，已知是在迁移的神经嵴，也分布在味蕾周围的组织隔室下的舌上皮，即，结缔组织核心的味觉乳头和von Ebner的腺体。通过使用SOX 10-Cre（一种由内源性SOX 10启动子驱动的Cremodel）与Cre报告细胞系R26-tdTomato（tdT）杂交对SOX 10表达细胞进行谱系追踪，我们在出生后小鼠的所有三种类型的味觉乳头（菌状、轮廓状和叶状）以及软腭中发现了SOX 10-Cre标记的tdT+细胞。tdT+味蕾细胞沿着发育阶段逐渐丰富，从出生时的几乎为零到成年时的35%以上。大多数tdT+味蕾细胞具有低强度的角蛋白8（广泛使用的味蕾细胞标记物）的免疫信号。在轮廓味蕾，tdT信号共定位主要与III型味蕾细胞标记，所以与I型和II型细胞标记。总之，我们的数据证明了一种新的祖细胞来源的味蕾出生后的小鼠-SOX 10-Cre标记的细胞在结缔组织核心和/或von Ebner的腺体。
Taste bud cells are specialized epithelial cells that undergo continuous turnover, and thus require active progenitors for their renewal and an intact taste function. Our previous studies suggested that a population of taste bud cells originates from outside of the surrounding tongue epithelium—previously regarded sole source of taste bud progenitors. In this study, we demonstrated thatSOX10(SRY-related HMG-box gene 10)-expressing cells, known to be in the migrating neural crest, were also distributed in taste bud-surrounding tissue compartments under the tongue epithelium, that is, the connective tissue core of taste papillae and von Ebner's glands. By lineage tracing ofSOX10-expressing cells usingSOX10-Cre, aCremodel driven by the endogenousSOX10promoter, crossing with aCrereporter line R26-tdTomato (tdT), we foundSOX10-Cre-labeled tdT+cells within taste buds in all three types of taste papillae (fungiform, circumvallate, and foliate) as well as in the soft palate in postnatal mice. The tdT+taste bud cells were progressively more abundant along the developmental stages, from virtually zero at birth to over 35% in adults. Most of tdT+taste bud cells had a low intensity of immunosignals of Keratin 8 (a widely used taste bud cell marker). In circumvallate taste buds, tdT signals were co-localized principally with a type III taste bud cell marker, less so with type I and II cell makers. Together, our data demonstrate a novel progenitor source for taste buds of postnatal mice—SOX10-Cre-labeled cells in the connective tissue core and/or von Ebner's glands.