Preparative Purification of Bioactive Compounds from Flos Chrysanthemi Indici and Evaluation of Its Antiosteoporosis Effect
Preparative Purification of Bioactive Compounds from Flos Chrysanthemi Indici and Evaluation of Its Antiosteoporosis Effect
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野菊花生物活性成分的制备纯化及其抗骨质疏松作用评价
DOI：
10.1155/2016/2587201
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发表时间：
2016-01-01
影响因子：
--
通讯作者：
Su, Jiansheng
中科院分区：
文献类型：
--
作者：
Li, Jia;Lin, Xiaosheng;Su, Jiansheng
为了解野菊花抗骨质疏松作用的物质基础和分子机制，评价野菊花乙醇提取物对卵巢切除（OVX）所致小鼠骨丢失的影响。此外，从FCI乙醇提取物中获得的抗骨质疏松级分使用制备型高速逆流色谱法（HSCCC）进行分离和纯化。研究了化合物对成骨细胞增殖和分化的体外影响。结果表明，乙酸乙酯部位具有较强的抗骨质疏松活性。采用梯度洗脱系统，HSCCC纯化的重要化合物包括金合欢素、芹菜素、木犀草素和蒙花苷。四种化合物均能促进MC 3 T3-E1细胞的成骨细胞分化和增殖。它们还增加了侏儒相关转录因子2（Runx 2）、骨钙素（OCN）、骨桥蛋白（OPN）和I型胶原（COL I）的mRNA水平。这四种化合物也激活了MC 3 T3-E1细胞中的AKT信号通路。本研究表明FCI的抗骨质疏松作用不依赖于单一组分，HSCCC能有效地从FCI中分离纯化抗骨质疏松活性成分。
To understand the material basis and underlying molecular machinery of antiosteoporosis activity of the Flos Chrysanthemi Indici (FCI), the consequences of ethanol extract on the bone loss in mice induced due to ovariectomy (OVX) was evaluated. Also, the antiosteoporosis fraction obtained from the FCI ethanol extract was isolated and purified using a preparative high-speed countercurrent chromatography (HSCCC). The in vitro impact of the compounds was investigated on osteoblast proliferation and differentiation. The results revealed that ethyl acetate fraction with robust in vivo antiosteoporosis activity was obtained. The important compounds purified by HSCCC using gradient elution system included acacetin, apigenin, luteolin, and linarin. The four compounds enhanced the differentiation and proliferation of osteoblasts in MC3T3-E1 cells. They also augmented the mRNA levels of runt-related transcription factor 2 (Runx2), osteocalcin (OCN), osteopontin (OPN), and type I collagen (COL I). The AKT signaling pathway was also activated in MC3T3-E1 cells by the four compounds. The present study demonstrated that the antiosteoporosis effects of FCI did not depend on a single component, and HSCCC efficiently isolated and purified the antiosteoporosis bioactive compounds from FCI.