Native mass spectrometry: a bridge between interactomics and structural biology
Native mass spectrometry: a bridge between interactomics and structural biology
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DOI：
10.1038/nmeth.1265
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发表时间：
2008-11-01
期刊：
影响因子：
48
通讯作者：
Heck, Albert J. R.
中科院分区：
文献类型：
--
作者：
Heck, Albert J. R.
天然质谱学是一项新兴的技术，它可以对完整的蛋白质络合物进行高灵敏度和理论上不受限制的质量范围的拓扑研究。这一独特的工具为结构生物学中已有的技术提供了补充信息，还提供了到高通量交互作用研究的链接，这些研究不会产生关于蛋白质复合体的确切组成、结构或动力学的信息。在这里，我回顾了国内质谱学技术的现状，并讨论了几个重要的生物学应用。我还描述了目前在自然质谱学方面的实验挑战，鼓励读者为解决方案做出贡献。
Native mass spectrometry is an emerging technology that allows the topological investigation of intact protein complexes with high sensitivity and a theoretically unrestricted mass range. This unique tool provides complementary information to established technologies in structural biology, and also provides a link to high-throughput interactomics studies, which do not generate information on exact protein complex-composition, structure or dynamics. Here I review the current state of native mass spectrometry technology and discuss several important biological applications. I also describe current experimental challenges in native mass spectrometry, encouraging readers to contribute to solutions.