Triterpenoid saponins from the rhizomes of Anemone flaccida and their inhibitory activities on LPS-induced NO production in macrophage RAW264.7 cells
Triterpenoid saponins from the rhizomes of Anemone flaccida and their inhibitory activities on LPS-induced NO production in macrophage RAW264.7 cells
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松莲花根茎中的三萜皂苷及其对 LPS 诱导巨噬细胞 RAW264.7 细胞 NO 产生的抑制活性
DOI：
10.1080/10286020.2014.954554
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发表时间：
2014-01-01
影响因子：
1.7
通讯作者：
Ye, Wen-Cai
中科院分区：
文献类型：
--
作者：
Huang, Xiao-Jun;Tang, Jing-Qun;Ye, Wen-Cai
从银莲花的根茎中分离得到一个新的熊果烷型三萜皂苷flaccidoside IV(1)和3个新的齐墩果烷型三萜皂苷flaccidoside V-vii(2-4)和17个已知皂苷(5-21)。通过光谱分析和化学方法确定了新的三萜皂苷的结构。对分离得到的皂苷在RAW264.7巨噬细胞中抑制脂多糖诱导的一氧化氮产生的活性进行了测试，发现几个双键链齐墩果型三萜皂苷(2、7和10)具有显著的抑制活性，表明它们在体外非细胞毒性浓度下具有潜在的抗炎活性。
A new ursane-type triterpenoid saponin, flaccidoside IV (1), and three new oleanane-type triterpenoid saponins, flaccidosides V-VII (2-4), along with 17 known saponins (5-21), were isolated from the rhizomes of Anemone flaccida. The structures of the new triterpenoid saponins were determined based on spectroscopic analyses and chemical methods. All the isolated saponins were tested for their inhibitory activities on lipopolysaccharide-induced nitric oxide production in RAW264.7 macrophages, and several bisdesmosidic oleanane-type triterpenoid saponins (2, 7, and 10) showed significant inhibitory activities, which indicated they had potential anti-inflammatory activities under their noncytotoxic concentrations in vitro.