Sodium fluoride induces apoptosis in cultured splenic lymphocytes from mice.
Sodium fluoride induces apoptosis in cultured splenic lymphocytes from mice.
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DOI：
10.18632/oncotarget.12081
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发表时间：
2016-10-18
期刊：
影响因子：
--
通讯作者：
Zhao L
中科院分区：
文献类型：
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作者：
Deng H;Kuang P;Cui H;Chen L;Fang J;Zuo Z;Deng J;Wang X;Zhao L
虽然氟在体内可诱导免疫器官的凋亡，但在体外培养的淋巴细胞中诱导凋亡的研究尚未见报道。本研究采用流式细胞术、免疫印迹和Hoechst 33258染色等方法，探讨氟化钠（NaF）诱导小鼠脾淋巴细胞凋亡及其机制。取3周龄雄性ICR小鼠脾淋巴细胞，体外暴露于NaF（0、100、200和400 μmol/L）24和48 h。流式细胞仪检测和Hoechst 33258染色结果显示，NaF诱导淋巴细胞凋亡，并通过降低线粒体跨膜电位，上调Bax、巴克、Fas、FasL、caspase 9、caspase 8、caspase 7、caspase 6和caspase 3蛋白表达而促进细胞凋亡Bcl-2和Bcl-xL蛋白表达下调（P <0.05或P <0.01）。上述结果提示氟化钠诱导脾淋巴细胞凋亡可能是通过线粒体途径和死亡受体途径介导的。
Though fluorine has been shown to induce apoptosis in immune organs in vivo, there has no report on fluoride-induced apoptosis in the cultured lymphocytes. Therefore, this study was conducted with objective of investigating apoptosis induced by sodium fluoride (NaF) and the mechanism behind that in the cultured splenic lymphocytes by flow cytometry, western blot and Hoechst 33258 staining. The splenic lymphocytes were isolated from 3 weeks old male ICR mice and exposed to NaF (0, 100, 200, and 400 μmol/L) in vitro for 24 and 48 h. When compared to control group, flow cytometry assay and Hoechst 33258 staining showed that NaF induced lymphocytes apoptosis, which was promoted by decrease of mitochondria transmembrane potential, up-regulation of Bax, Bak, Fas, FasL, caspase 9, caspase 8, caspase 7, caspase 6 and caspase 3 protein expression (P < 0.05 or P <0.01), and down-regulation of Bcl-2 and Bcl-xL protein expression (P <0.05 or P <0.01). The above-mentioned data suggested that NaF-induced apoptosis in splenic lymphocytes could be mediated by mitochondrial and death receptor pathways.