GENETIC INTERACTIONS AND DOSAGE EFFECTS OF POLYCOMB GROUP GENES OF DROSOPHILA
GENETIC INTERACTIONS AND DOSAGE EFFECTS OF POLYCOMB GROUP GENES OF DROSOPHILA
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DOI：
10.1007/bf00288601
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发表时间：
1995-02-06
期刊：
影响因子：
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通讯作者：
BROCK, HW
中科院分区：
文献类型：
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作者：
CAMPBELL, RB;SINCLAIR, DAR;BROCK, HW
Polycomb（Pc）基因组是维持果蝇细胞决定所必需的。至少有11个Pc组基因已被描述，可能有多达40个;所有的同源异型基因的正常调节所需的，但作为一个组，它们的表型是相当多样化的。有人认为，Pc组基因的产物可能是异聚体复合物的成员，其作用是调节靶基因座的染色质结构。我们研究了成年苍蝇的表型杂合子的每一个成对的组合的Pc组基因，试图细分的Pc组功能。这些结果支持了附加性梳（Asx）、多梳样性梳（Pcl）、后性梳（Psc）、足中部性梳（Scm）和额外性梳（Sce）在某些组织中具有相似功能的观点。我们显示了额外的性梳（ESC）和Asx，增强Pc，Pcl，增强zeste E（z），和超级性梳之间的遗传相互作用，并重新评估大多数Pc组基因的功能独立于ESC的想法。大多数重复的Pc组基因既不表现出前向转化，也不抑制额外的性梳表型的Pc组突变，这表明并不是所有的Pc组基因的行为所预测的大规模行动模型。令人惊讶的是，E（z）的重复增强了esc突变体的同源异型表型。果蝇在esc（+）剂量增加的情况下表现出前向转化，但这些转化并不因tr三胸组基因突变而增强。结果进行了讨论，相对于目前的模型Pc组功能。
The Polycomb (Pc) group of genes are required for maintenance of cell determination in Drosophila melanogaster. At least 11 Pc group genes have been described and there may be up to 40; all are required for normal regulation of homeotic genes, but as a group, their phenotypes are rather diverse. It has been suggested that the products of Pc group genes might be members of a heteromeric complex that acts to regulate the chromatin structure of target loci. We examined the phenotypes of adult flies heterozygous for every pairwise combination of Pc group genes in an attempt to subdivide the Pc group functionally. The results support the idea that Additional sex combs (Asx), Pc, Polycomblike (Pcl), Posteriol sex combs (Psc), Sex combs on midleg (Scm), and Sex combs extra (Sce) have similar functions in some imaginal tissues. We show genetic interactions among extra sex combs (esc) and Asx, Enhancer of Pc, Pcl, Enhancer of zeste E(z), and super sex combs and reassess the idea that most Pc group genes function independently of esc. Most duplications of Pc group genes neither exhibit anterior transformations nor suppress the extra sex comb phenotype of Pc group mutations, suggesting that not all Pc group genes behave as predicted by the mass-action model. Surprisingly, duplications of E(z) enhance homeotic phenotypes of esc mutants. Flies with increasing doses of esc(+) exhibit anterior transformations, but these are not enhanced by mutations in tr trithorax group genes. The results are discussed with respect to current models of Pc group function.