Porous microcapsules encapsulating β cells generated by microfluidic electrospray technology for diabetes treatment
Porous microcapsules encapsulating β cells generated by microfluidic electrospray technology for diabetes treatment
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DOI：
10.1038/s41427-022-00385-5
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发表时间：
2022-12-01
影响因子：
9.7
通讯作者：
Li, Ling
中科院分区：
文献类型：
--
作者：
Liu, Xiaoyu;Yu, Yunru;Li, Ling
糖尿病在世界范围内日益流行，需要有效的临床治疗方法。近年来，β-细胞替代疗法已成为糖尿病治疗的安全替代方案，并且已提出包封方法来促进这种类型的疗法。在这里，我们使用同轴微流体电喷雾技术在不到半小时的时间内产生具有多孔藻酸盐壳和含β细胞核的高细胞活力（>90%）的微胶囊。利用微流控电喷雾技术，制备的微胶囊尺寸可调。生物相容性多孔水凝胶壳不仅保护β细胞免受免疫排斥，而且允许在移植过程中交换小分子营养物质，并且液体核心保证了包封细胞的高活力。这种构建的活细胞生物系统在移植到糖尿病小鼠的网膜袋中以控制血糖水平从而治疗糖尿病后进一步证明了其作为人工胰岛的潜力。我们认为，该系统具有精心设计的结构和丰富的高活性包囊β细胞，以提高治疗性能，可以应用于广泛的临床情况。
Diabetes mellitus is becoming increasingly prevalent worldwide and needs effective clinical treatment methods. beta-Cell replacement therapy has become a safe alternative for diabetes treatment in recent years, and encapsulation methods have been proposed to facilitate this type of therapy. Here, we used coaxial microfluidic electrospray technology to generate microcapsules allowing high cell viability (>90%) with porous alginate shells and beta cell-containing cores in less than half an hour. Benefitting from microfluidic electrospray, the sizes of the generated microcapsules were adjustable. The biocompatible porous hydrogel shell not only protected beta cells from immune rejection but also allowed the exchange of small molecular nutrients during transplantation, and the liquid core guaranteed the high viability of the encapsulated cells. This constructed living cell biosystem further demonstrated its potential as an artificial islet after transplantation into the omental pouches of diabetic mice to control blood glucose levels and thus treat diabetes. We consider that this system, with an elaborate structure and an abundance of highly viable encapsulated beta cells to improve treatment performance, could be applied in a wide range of clinical situations.