Maximizing DNA loading on a range of gold nanoparticle sizes
Maximizing DNA loading on a range of gold nanoparticle sizes
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DOI：
10.1021/ac0613582
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发表时间：
2006-12-15
影响因子：
7.4
通讯作者：
Mirkin, Chad A.
中科院分区：
文献类型：
--
作者：
Hurst, Sarah J.;Lytton-Jean, Abigail K. R.;Mirkin, Chad A.
我们研究了影响金纳米颗粒 DNA 覆盖度的变量。已经评估了盐浓度、间隔基组成、纳米颗粒尺寸和超声处理程度的影响。在含有聚乙二醇间隔基的 DNA 存在下，通过将纳米颗粒盐老化至类似于 0.7 M NaCl 来获得最大负载。此外，通过在表面加载过程中对纳米粒子进行超声处理，DNA 加载量显着增加。最后，发现直径达 250 nm 的纳米颗粒比较小（13-30 nm）的纳米颗粒的 DNA 负载量高出大约 2 个数量级，这是由于其表面积较大的结果。稳定的大颗粒对于各种生物诊断测定都很有吸引力。
We have investigated the variables that influence DNA coverage on gold nanoparticles. The effects of salt concentration, spacer composition, nanoparticle size, and degree of sonication have been evaluated. Maximum loading was obtained by salt aging the nanoparticles to similar to 0.7 M NaCl in the presence of DNA containing a poly( ethylene glycol) spacer. In addition, DNA loading was substantially increased by sonicating the nanoparticles during the surface loading process. Last, nanoparticles up to 250 nm in diameter were found have similar to 2 orders of magnitude higher DNA loading than smaller (13-30 nm) nanoparticles, a consequence of their larger surface area. Stable large particles are attractive for a variety of biodiagnostic assays.