Identification of new genetic subtypes of bovine viral diarrhea virus genotype 1 isolated in Japan
Identification of new genetic subtypes of bovine viral diarrhea virus genotype 1 isolated in Japan
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DOI：
10.1007/s11262-007-0190-0
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发表时间：
2008-02-01
期刊：
影响因子：
1.6
通讯作者：
Sakoda, Yoshihiro
中科院分区：
文献类型：
--
作者：
Nagai, Makoto;Hayashi, Michiko;Sakoda, Yoshihiro
测定了属于牛病毒性腹泻病毒基因型1(BVDV-1)的So-like分离株和IS25CP/01株的5'非翻译区(5'UTR)和E-rns区的部分核苷酸序列以及编码N-pro、Core和E2的基因组区域，并与其他BVDV亚基因型的序列进行了遗传比较。使用 5'UTR 和 N-pro 进行的系统发育分析表明，So 样分离株和 IS25CP/01 分支成独立的系统发育分支。 So-like 分离株与取自 DDBL/EMBL/GenBank 5'UTR 的韩国 BVDV 毒株聚集在一起。在 IS25CP/01 核心区的 C 末端发现了另外两个氨基酸残基。 So样分离株E2和IS25CP/01与BVDV-1参考株NADL的氨基酸序列相似性分别为78.0-78.5和79.0。交叉中和试验显示So-like分离株与其他分离株（抗原相似性（R）值：2.2-8.8）、IS25CP/01与其他分离株（R值：1.6-8.8）之间存在显着的抗原差异。 So-like病毒和IS25CP/01与Jackova等人报告的第13种亚基因型（1a-1m）不同。 (2007) 并被分类为新的遗传亚型 BVDV-1n（So 样）和 1o (IS25CP/01)。
A part of the nucleotide sequence of the 5' untranslated region (5'UTR) and E-rns region, and the genomic regions encoding for N-pro, Core, and E2 of So-like isolates and IS25CP/01 strain which belong to bovine viral diarrhea virus genotype 1 (BVDV-1) were determined and genetic comparisons were made with sequences of other BVDV subgenotypes. Phylogenetic analysis using the 5'UTR and N-pro revealed that So-like isolates and IS25CP/01 branched into independent phylogenetic branch. So-like isolates were clustered with Korean BVDV strains taken from DDBL/EMBL/GenBank in the 5'UTR. An additional two amino acid residues were found at the C terminal of the Core region of IS25CP/01. The similarity of amino acid sequence of E2 of So-like isolates and IS25CP/01 to the BVDV-1 reference strain NADL were 78.0-78.5 and 79.0, respectively. Cross-neutralization tests showed significant antigenic differences between So-like isolates and the others (Antigenic similarity (R) value: 2.2-8.8), and IS25CP/01 and the others (R value: 1.6-8.8). So-like viruses and IS25CP/01 differed from the thirteenth subgenotypes (1a-1m) reported by Jackova et al. (2007) and were classified as new genetic subtypes, BVDV-1n (So-like) and 1o (IS25CP/01).