Label-free dynamic imaging of mitochondria and lysosomes within living cells via simultaneous dual-pump photothermal microscopy
Label-free dynamic imaging of mitochondria and lysosomes within living cells via simultaneous dual-pump photothermal microscopy
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DOI：
10.1364/boe.10.005852
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发表时间：
2019-11-01
影响因子：
3.4
通讯作者：
Toumon, Yasunobu
中科院分区：
文献类型：
--
作者：
Miyazaki, Jun;Toumon, Yasunobu
利用高灵敏度光热显微镜（PT）观察了线粒体和溶酶体的动态活性，它们在维持细胞稳态中起重要作用。这种成像方式允许直接观察含有内源性发色团的细胞器，具有高时间和空间分辨率。我们通过同时双色成像鉴定了活体哺乳动物细胞内的线粒体和溶酶体。此外，动态成像显示溶酶体与线粒体接触并在动态线粒体网络内的位点之间移动。由于线粒体和溶酶体的功能错综复杂，PT显微镜应该提供深入了解与线粒体-溶酶体通讯相关的细胞功能，以及由这些细胞器功能障碍引起的各种人类疾病的见解。(C) 2019年美国光学学会根据OSA开放获取出版协议的条款
The dynamic activities of mitochondria and lysosomes, which play important roles in maintaining cellular homeostasis, were observed without labeling by using highly sensitive photothermal (PT) microscopy. This imaging modality allows for the direct observation of cellular organelles that contain endogenous chromophores, with high temporal and spatial resolution. We identified mitochondria and lysosomes inside living mammalian cells via simultaneous dual-color imaging. Moreover, dynamic imaging revealed that the lysosomes make contact with mitochondria and move between sites within the dynamic mitochondria) network. Since mitochondrial and lysosomal functions are intricately connected, PT microscopy should provide in-depth understanding of cellular functions associated with mitochondria-lysosome communication as well as insights into various human diseases caused by dysfunction of these organelles. (C) 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement