Dynamics of keratin filaments and the intermediate filament distribution center during shape change in PtK1 cells.
Dynamics of keratin filaments and the intermediate filament distribution center during shape change in PtK1 cells.
复制标题
PtK1 细胞形状变化过程中角蛋白丝和中间丝分布中心的动态。
DOI：
10.1002/cm.970040303
复制
发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Warren,RH
中科院分区：
文献类型：
--
作者：
Eckert,BS;Caputi,SE;Warren,RH
通过免疫荧光、电子显微镜和延时视频显微镜检查细胞扩散过程中中间丝的重组。当细胞扩散时，观察到一个被认为对应于中间丝分布中心的核旁帽在空间上与中间丝网络的组织相关。观察到角蛋白帽在活动的 PtK1 细胞中自发出现。帽的形成可能是细胞移动时中间丝从细胞质中回缩的结果。该近核帽的位置与运动方向相关，位于靠近细胞前进边缘的细胞核一侧。随着细胞扩散，角蛋白丝网络返回细胞质，帽消失。这里提供的证据与分布中心在细胞形状变化过程中介导角蛋白丝组织的假设是一致的。
Reorganization of intermediate filaments during cell spreading is examined by immunofluorescence, electron microscopy, and time‐lapse video microscopy. A juxtanuclear cap, believed to correspond to the intermediate filament distribution center, was observed to be spatially related to the organization of the intermediate filament network as cells spread. A keratin cap was observed, which appeared spontaneously in motile PtK1 cells. Cap formation may be a consequence of retraction of intermediate filaments from the cytoplasm as cells move. The position of this juxtanuclear cap is related to the direction of movement, located on the side of the nucleus near the advancing edge of the cell. As the cell spreads, the cap disappears as the keratin filament network returns to the cytoplasm. Evidence presented here is consistent with the hypothesis that the distribution center mediates keratin filament organization during cell shape change.