Herpes simplex virus glycoproteins gC-1 and gC-2 bind to the third component of complement and provide protection against complement- mediated neutralization of viral infectivity
Herpes simplex virus glycoproteins gC-1 and gC-2 bind to the third component of complement and provide protection against complement- mediated neutralization of viral infectivity
复制标题
单纯疱疹病毒糖蛋白 gC-1 和 gC-2 与补体的第三个成分结合，并提供针对补体介导的病毒感染性中和的保护
DOI：
--
复制
发表时间：
1987
影响因子：
15.3
通讯作者：
John P. Atkinson
中科院分区：
文献类型：
--
作者：
T. Mcnearney;Christine Odell;V. Holers;Patricia G. Spear;John P. Atkinson
单纯疱疹病毒1型（HSV-1）感染的细胞与C3 b包被的红细胞形成红细胞，而单纯疱疹病毒2型（HSV-2）或其他疱疹病毒感染的细胞则不会。据报道，HSV-1的糖蛋白C（gC-1）介导C3 b包被的红细胞与感染细胞的结合，并对人补体的旁路途径C3转化酶具有调节（衰变加速）活性。我们在这里显示，溶解的gC-1结合到iC 3-琼脂糖亲和柱。我们还报告了增溶的gC-2，由HSV-2指定的遗传相关糖蛋白，结合到iC 3-琼脂糖。使用gC-1或gC-2和突变病毒株特异性mAb鉴定C3结合糖蛋白。在其他实验中，在存在或不存在HSV gD特异性抗体的情况下，测试HSV-1突变株和重组体（仅在其gC表达方面不同）对人补体中和的敏感性。在这两种情况下，gC-菌株最敏感。通过同基因插入突变体表达gC-1或gC-2提供了针对补体介导的中和的保护。这些结果表明，在遗传和结构上相关的gC-1和gC-2共享与人C3结合的功能活性并增强病毒感染性。
Cells infected with herpes simplex virus type 1 (HSV-1) form rosettes with C3b-coated erythrocytes, whereas cells infected with herpes simplex virus type 2 (HSV-2) or other herpes viruses do not. It was reported that glycoprotein C of HSV-1 (gC-1) mediates the binding of C3b-coated erythrocytes to infected cells and has regulatory (decay- accelerating) activity for the alternative pathway C3 convertase of human complement. We show here that solubilized gC-1 binds to iC3- Sepharose affinity columns. We also report that solubilized gC-2, the genetically related glycoprotein specified by HSV-2, binds to iC3- Sepharose. mAb specific for gC-1 or gC-2 and mutant viral strains were used to identify the C3-binding glycoproteins. In other experiments, HSV-1 mutant strains and recombinants, differing only in their expression of gC, were tested for sensitivity to neutralization by human complement in the presence or absence of antibodies specific for HSV gD. In either case the gC- strain was most sensitive. Expression of gC-1 or gC-2 by isogenic insertion mutants provided protection against complement-mediated neutralization. These results indicate that the genetically and structurally related gC-1 and gC-2 share the functional activity of binding to human C3 and enhance viral infectivity.
内皮细胞、上皮细胞和成纤维细胞的 1 型单纯疱疹病毒感染会诱导 C3b 受体。
DOI：
--
发表时间：
1985
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Smiley,ML;Hoxie,JA;Friedman,HM
通讯作者：
Friedman,HM
单纯疱疹病毒 1 的糖蛋白 C 是补体级联的抑制剂。
DOI：
--
发表时间：
1986
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Fries,LF;Friedman,HM;Cohen,GH;Eisenberg,RJ;Hammer,CH;Frank,MM
通讯作者：
Frank,MM