Isolation of mutants with aberrant mitochondrial morphology from Arabidopsis thaliana
Isolation of mutants with aberrant mitochondrial morphology from Arabidopsis thaliana
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DOI：
10.1266/ggs.79.301
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发表时间：
2004-10-01
影响因子：
1.1
通讯作者：
Tsutsumi, N
中科院分区：
文献类型：
--
作者：
Feng, XG;Arimura, S;Tsutsumi, N
为了鉴定与植物线粒体形态和动力学相关的基因，从甲基磺酸乙酯（EMS）突变的拟南芥种群中分离出线粒体形态的新突变体。通过用由GFP和靶向拟南芥的前序列组成的融合蛋白的基因转化拟南芥来可视化线粒体。从19，000个M2群体中，通过荧光显微镜观察分离到17个突变体。这些突变体中的所有线粒体都比野生型线粒体更长和/或更大。确定了7个生长良好的突变体的突变的近似染色体位点。其中6个突变体的线粒体表型为隐性，而第7个突变体的线粒体表型为显性。对7个突变体进行染色体定位，发现突变发生在4个不同的位点。这些位点中至少有一个是新的，即，该位点与已知的拟南芥线粒体形态突变体的位点不同，也不同于与线粒体形态相关的酵母基因的拟南芥同源物的位点。
To identify genes related to plant mitochondrial morphology and dynamics, novel mutants with respect to mitochondrial morphology were isolated from an ethyl methane sulphonate (EMS)-mutated population of Arabidopsis thaliana. Mitochondria were visualized by transforming Arabidopsis with a gene for a fusion protein consisting of GFP and a mitochondria-targeting pre-sequence. From 19,000 M2 populations, 17 mutants were isolated by fluorescent microscopic observations. All mitochondria in these mutants were longer and/or larger than wild-type mitochondria. The approximate chromosomal loci of the mutations of seven mutants that grew well were determined. The mitochondrial phenotypes of six of the mutants were recessive but the mitochondrial phenotype of the seventh mutant was dominant. Chromosomal rough mapping of the seven mutants showed that the mutations occurred at four different loci. At least one of these loci was novel, i.e., it was different from loci of other known mitochondrial morphology mutants of Arabidopsis and different from loci of Arabidopsis homologues of yeast genes related to mitochondrial morphology.