Phylogeny of the genus Flavivirus using complete coding sequences of arthropod-borne viruses and viruses with no known vector
Phylogeny of the genus Flavivirus using complete coding sequences of arthropod-borne viruses and viruses with no known vector
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DOI：
10.1099/0022-1317-81-3-781
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发表时间：
2000-03-01
影响因子：
3.8
通讯作者：
de Lamballerie, X
中科院分区：
文献类型：
--
作者：
Billoir, F;de Chesse, R;de Lamballerie, X
由于缺乏遗传信息，尤其是非载体黄病毒中的遗传信息，定义黄病毒属病毒的进化关系和起源的尝试受到阻碍。使用新的序列测定方案，报告了圣路易斯脑炎病毒和两种代表性非载体黄病毒Pie Brave（从蝙蝠中分离）和Apoi（从啮齿动物中分离）的第一个完整编码序列，Rio Brave和Apoi病毒的编码多蛋白是黄病毒属中迄今为止描述的最小的，与其他黄病毒具有最高的相似性 存在于 NS3 和 NS5 基因中。迄今为止已完全测序的黄病毒中，病毒丝氨酸蛋白酶的蛋白水解切割位点高度保守。比较遗传氨基酸比对显示，p 距离截止值 0.330-0.470 根据节肢动物传播的病毒所识别的血清群来区分节肢动物传播的病毒，而 Rio Brave 和 Apoi 病毒被分配到两个不同的非载体病毒组。在这些血清群中，基于完整 ORF 序列的分支发生与基于包膜和 NS5 序列的树相似。相比之下，树较深层节点的分支模式与之前 NS5 序列研究中报道的不同。讨论了这些观察结果的重要性。
Attempts to define the evolutionary relationships and origins of viruses in the genus Flavivirus are hampered by the lack of genetic information particularly amongst the non-vectored flaviviruses. Using a novel protocol for sequence determination, the first complete coding sequence of St Louis encephalitis virus and those of two representative non-vectored flaviviruses, pie Brave (isolated from bat) and Apoi (isolated from rodent), are reported, The encoded polyproteins of Rio Brave and Apoi virus are the smallest described to date within the genus Flavivirus, The highest similarities with other flaviviruses were found in the NS3 and NS5 genes. The proteolytic cleavage sites for the viral serine protease were highly conserved among the flaviviruses completely sequenced to date. Comparative genetic amino acid alignments revealed that p-distance cut-off values of 0.330-0.470 distinguished the arthropod-borne viruses according to their recognized serogroups and Rio Brave and Apoi virus were assigned to two distinct non-vectored virus groups. Within these serogroups, cladogenesis based on the complete ORF sequence was similar to trees based on envelope and NS5 sequences. In contrast, branching patterns at the deeper nodes of the tree were different from those reported in the previous study of NS5 sequences. The significance of these observations is discussed.