DNA shuffling and vaccines.
DNA shuffling and vaccines.
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DOI：
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发表时间：
2001-02
影响因子：
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通讯作者：
R. Whalen;R. Kaiwar;N. Soong;J. Punnonen
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文献类型：
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作者：
R. Whalen;R. Kaiwar;N. Soong;J. Punnonen
生物技术的一个挑战是找到在自然界中通常找不到的新环境中优化酶、细胞因子、疫苗或转基因的方法。DNA改组的方法是一种试管过程，利用重组产生嵌合基因文库，然后可以筛选这些嵌合基因文库以鉴定在一种或多种功能上改进的编码蛋白质。结构相似且功能同源的两个或更多个基因的DNA改组在产生功能新颖分子的大型文库中特别有效。其他定向进化方法，如涉及定向或随机诱变的那些，与DNA改组重组过程相比具有若干限制。各种各样的基因已提交DNA改组，并已获得各种功能参数的显着改善。这里讨论了DNA改组在疫苗开发、治疗和基因治疗中的应用的几个例子。
One challenge of biotechnology is to find ways to optimize enzymes, cytokines, vaccines or transgenes in new contexts that are typically not found in nature. The approach of DNA shuffling is a test-tube process that takes advantage of recombination to generate libraries of chimeric genes, which can then be screened to identify the encoded proteins improved in one or more functions. DNA shuffling of two or more genes that are structurally similar and homologous in function is particularly efficacious in generating large libraries of functionally novel molecules. Other directed evolution methods, such as those involving directed or random mutagenesis, have several limitations compared to the DNA shuffling recombination process. A wide variety of genes have been submitted to DNA shuffling, and significant improvements in various functional parameters have been obtained. Several examples of the application of DNA shuffling to vaccine development, therapeutics and gene therapy are discussed here.