Microfluidic particle sorter employing flow splitting and recombining
Microfluidic particle sorter employing flow splitting and recombining
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DOI：
10.1021/ac0520083
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发表时间：
2006-02-15
影响因子：
7.4
通讯作者：
Seki, M
中科院分区：
文献类型：
--
作者：
Yamada, M;Seki, M
本文描述了一种改进的微流控装置，使流体动力学颗粒浓度和大小依赖分离能够连续进行。在我们之前的研究中，我们提出了一种利用具有多个分支点和侧通道的微通道对颗粒进行水动力过滤和分选的方法，并将其用于聚合物颗粒和细胞的连续浓缩和分离。在目前的研究中，通过对主流流体的几何分离和重组，大大提高了颗粒分选的效率。通过这些操作，直径大于特定值的颗粒在主流中向一侧侧壁移动。这种对颗粒位置的控制之后是颗粒在侧壁上的完美排列，这增加了选择性和回收率，而无需使用不含颗粒的液体。在本研究中，设计并制作了一个有一个入口和五个出口的微通道。通过简单地将颗粒悬浮液引入该装置，将2.1-3.0 μ m颗粒的浓度提高60 - 80倍，并从每个出口独立收集。此外，当将微通道网络视为电阻电路时，分布在各侧通道的测量流量与理论值吻合良好。
This paper describes an improved microfluidic device that enables hydrodynamic particle concentration and size-dependent separation to be carried out in a continuous manner. In our previous study, a method for hydrodynamic filtration and sorting of particles was proposed using a microchannel having multiple branch points and side channels, and it was applied for continuous concentration and separation of polymer particles and cells. In the current study, the efficiency of particle sorting was dramatically improved by geometrically splitting fluid flow from a main stream and recombining. With these operations, particles with diameters larger than a specific value move toward one sidewall in the mainstream. This control of particle positions is followed by the perfect particle alignment onto the sidewall, which increases the selectivity and recovery rates without using a liquid that does not contain particles. In this study, a microchannel having one inlet and five outlets was designed and fabricated. By simply introducing particle suspension into the device, concentrations of 2.1-3.0-mu m particles were increased 60 - 80-fold, and they were collected independently from each outlet. In addition, it was demonstrated that the measured flow rates distributed into each side channel corresponded well to the theoretical values when regarding the microchannel network as a resistive circuit.