STUDIES IN VITRO OF PASSAGE OF SERUM PROTEINS ACROSS INTESTINAL WALL OF YOUNG RATS
STUDIES IN VITRO OF PASSAGE OF SERUM PROTEINS ACROSS INTESTINAL WALL OF YOUNG RATS
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DOI：
10.1098/rspb.1966.0083
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发表时间：
1966-01-01
期刊：
影响因子：
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通讯作者：
BAMFORD, DR
中科院分区：
文献类型：
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作者：
BAMFORD, DR
使用 18 日龄大鼠的外翻回肠囊作为体外制剂，并描述了其制备的改进技术。这些囊在至少 1 小时内保持活力和功能。当在37℃有氧条件下孵育时。这些制剂显示，在实验期间，L-组氨酸或放射性碘的浓度梯度穿过肠道的转运和摄氧量是一致的。同源和异源凝集素和 I131 标记的 γ-球蛋白从粘膜转运至浆膜液[在白痢沙门氏菌攻击后]。丙种球蛋白从粘膜转移到浆膜液的方式不同，具体取决于来源的物种。吸收的蛋白质的一部分在运输过程中被分解，并且在蛋白质运输过程中组织的耗氧量增加。
Everted ileal sacs of 18-day-old rats were used as in vitro preparations, and a modified technique for their preparation is described. These sacs remained viable and functional for at least 1 hr. when incubated at 37[degree]C under aerobic conditions. Such preparations showed transport across the intestine against concentration gradients of L-histidine or of radioactive iodine and oxygen uptake was consistent over the period of the experiment. Homologous and heterologous agglutinins and I131-labelled [gamma]-globulins were transported from the mucosal to the serosal fluid [after challenge with Salmonella pullorum]. Gamma-globulins were transmitted from the mucosal to the serosal fluid differentially depending upon the species of origin. A part of the protein absorbed was broken down during transport and an increase in oxygen consumption of the tissues occurred during protein transport.