Nuclear magnetic resonance investigation of oriented lipid membranes.
Nuclear magnetic resonance investigation of oriented lipid membranes.
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定向脂质膜的核磁共振研究。
DOI：
10.1007/978-1-59745-519-0_6
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Gawrisch,Klaus
中科院分区：
文献类型：
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作者：
Soubias,Olivier;Gawrisch,Klaus
~(31)P和~2H-核磁共振被广泛用于研究固体界面上脂双层的有序性和动力学，以及脂双分子膜的取向。本文介绍了在界面上定向脂质双分子膜的各种技术、获取核磁共振波谱的要求以及波谱解释的基本原理。介绍了两种定向双层膜的制备方法：平面固体界面取向的多层双层膜和多孔衬底内的单层管状双层膜。后一种双层可以从散装水相进入，这允许调节pH，以及将蛋白质和配体输送到双层表面。
31P-and2H-nuclear magnetic resonance are widely used to study order and dynamics, but also orientation of lipid bilayers at solid interfaces. Herein, the various techniques of orienting lipid bilayers at interfaces, the requirements for acquisition of nuclear magnetic resonance spectra, and the basic principles for spectra interpretation are described. Preparation of two types of oriented bilayers is presented: multilamellar bilayers oriented at flat solid interfaces and unilamellar tubular bilayers inside porous substrates. The latter bilayers are accessible from a bulk water phase, which permits adjustment of pH, as well as delivery of proteins and ligands to the bilayer surface.