Isoimperatorin, cimiside E and 23-O-acetylshengmanol-3-xyloside from Cimicifugae Rhizome inhibit TNF-α-induced VCAM-1 expression in human endothelial cells: Involvement of PPAR-γ upregulation and PI3K, ERK1/2, and PKC signal pathways
Isoimperatorin, cimiside E and 23-O-acetylshengmanol-3-xyloside from Cimicifugae Rhizome inhibit TNF-α-induced VCAM-1 expression in human endothelial cells: Involvement of PPAR-γ upregulation and PI3K, ERK1/2, and PKC signal pathways
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DOI：
10.1016/j.jep.2010.10.004
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发表时间：
2011-01-27
影响因子：
5.4
通讯作者：
Chang, Ki Churl
中科院分区：
文献类型：
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作者：
Moon, Lidiya;Ha, Yu Mi;Chang, Ki Churl
民族药理学相关性：升麻根茎的甲醇提取物传统上用于各种疾病，包括炎症。研究目的：本研究的目的是探讨三种活性化合物、两种三萜苷类化合物是否具有抗炎作用（西米苷E、23-O-乙酰生烷醇-3-木糖苷）和一种呋喃香豆素异欧前胡素（isoimperatorin）是从升麻（Cimicifugae Rhizome）中分离得到的一种与过氧化物酶体增殖物激活受体-γ（peroxisome proliferator-activated receptor-γ）相关的化合物材料和方法：进行细胞活力和活性氧的产生。此外，粘附单核细胞进入内皮细胞和蛋白质印迹的粘附分子和信号蛋白的表达在肿瘤坏死因子-α（TNF-α）-activated cells.Results：预处理的测试化合物显着减少活性氧（ROS）的产生和表达的血管细胞粘附分子-1（VCAM-1），但不细胞间细胞粘附分子-1（ICAM-1）。三种化合物均剂量依赖性地增加EA.hy926细胞中的PPAR-gamma表达，但以不同的特异性抑制TNF-α诱导的Akt、细胞外信号调节激酶（ERK）和蛋白激酶C（PKC）的磷酸化。最后，他们阻止了TNF-α诱导的粘附U937单核细胞EA.hy926 cells.Conclusions：目前的结果表明，cimiside E，23-O-actylshengmanol-3-xyloside，isoimperatorin从升麻根茎分离选择性抑制TNF-α诱导的VCAM-1的表达至少通过上调的PPAR-gamma，和信号ERK 1/2，P13 K，和PKC参与了这种效果。(C)2010爱思唯尔爱尔兰有限公司版权所有。
Ethnopharmacological relevance: The methanol extract of Cimicifugae Rhizome has been traditionally used in various disorders including inflammation.Aim of the study: The aim of the study is to explore whether anti-inflammatory action of 3 active compounds, two triterpenoid glycosides (cimiside E, 23-O-actylshengmanol-3-xyloside) and one furanocoumarin (isoimperatorin), isolated from Cimicifugae Rhizome is related with peroxisome proliferator-activated receptor-gamma (PPAR-gamma) expression in human umbilical endothelial cell line, EA.hy926 cells.Materials and methods: Cell viability and production of reactive oxygen species were performed. In addition, adhesion of monocyte into endothelial cells and western blot for expression of adhesion molecules and signal proteins were investigated in tumor necrosis factor-alpha (TNF-alpha)-activated cells.Results: Pretreatment of test compounds significantly reduced reactive oxygen species (ROS) production and expression of vascular cell adhesion molecule-1 (VCAM-1), but not intercellular cell adhesion molecule-1 (ICAM-1). Three compounds all dose-dependently increased not only PPAR-gamma expression in EA.hy926 cells but inhibited TNF-alpha-induced phosphorylation of Akt, extracelullar-signal-regulated kinase (ERK) and protein kinase C (PKC) with different specificity. Finally, they prevented TNF-alpha-induced adhesion of U937 monocytic cells to EA.hy926 cells.Conclusions: The present results show that cimiside E, 23-O-actylshengmanol-3-xyloside, isoimperatorin isolated from Cimicifugae Rhizome selectively inhibits TNF-alpha-induced expression of VCAM-1 at least by upregulation of PPAR-gamma, and signals for ERK1/2, P13K, and PKC are involved in this effect. (C) 2010 Elsevier Ireland Ltd. All rights reserved.