DOSE-RELATED CARCINOGENIC EFFECTS OF WATER-BORNE BENZO[A]PYRENE ON LIVERS OF 2 SMALL FISH SPECIES
DOSE-RELATED CARCINOGENIC EFFECTS OF WATER-BORNE BENZO[A]PYRENE ON LIVERS OF 2 SMALL FISH SPECIES
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DOI：
10.1016/0147-6513(88)90052-8
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发表时间：
1988-12-01
影响因子：
6.8
通讯作者：
LYTLE, JS
中科院分区：
文献类型：
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作者：
HAWKINS, WE;WALKER, WW;LYTLE, JS
水中接触的苯并[a]芘（BaP）会对两种小型鱼类——日本青鳉鱼（Oryzias latipes）和孔雀鱼（Poecilia reticulata）的肝脏产生剂量相关的致癌作用。青鳉和孔雀鱼各接受两次 6 小时的暴露。第一次暴露是在 6 至 10 天大的样本上进行的。 7 天后进行第二次暴露。测试包含五个治疗组：(1) 对照组，(2) 载体（二甲基甲酰胺）对照组，(3) 低 BaP 剂量（检测不到.sbd.4 ppb），（4）中等 BaP 剂量（约 8-47 ppb BaP），以及（5）高 BaP 剂量（200-270 ppb）。高剂量暴露后，这两个物种都发生了被分类为细胞改变灶（改变灶）、腺瘤和肝细胞癌的肝细胞病变。在青鳉中，病变似乎是依次发展的，出现改变的病灶，然后是腺瘤，然后是肝细胞癌。然而，孔雀鱼的大多数病变被归类为改变的病灶，尽管早期（24周）样本中发生了一些腺瘤，而晚期（52周）样本中发生了肝细胞癌。当合并总病变时，初次暴露后 24 周时青鳉的发病率为 11%，36 周时发病率为 36%。在孔雀鱼中，8% 在 24 周时出现肝脏病变，19% 在 36 周时出现肝脏病变，20% 在 52 周时出现肝脏病变。 36 周高剂量样本中的青鳉体内出现了单一肝外肿瘤，即鳃丝中的毛细血管瘤。结果表明，青鳉和孔雀鱼能够将水中的 BaP 代谢为致癌代谢物，从而引发肝肿瘤的发展。
Benzo[a]pyrene (BaP) administered by water-borne exposures caused dose-related carcinogenic effects in livers of two small fish species, the Japanese medaka (Oryzias latipes) and the guppy (Poecilia reticulata). Medaka and guppies each were given two 6-h exposures. The first exposure was conducted on 6- to 10-day-old specimens. The second exposure was given 7 days later. The tests incorporated five treatment groups: (1) control, (2) carrier (dimethylformamide) control, (3) low BaP dose (not detectable.sbd.4 ppb), (4) intermediate BaP dose (about 8-47 ppb BaP), and (5) high BaP dose (200-270 ppb). Following the high-dose exposure, heptocellular lesions classified as foci of cellular alteration (altered foci), adenomas, and hepatocellular carcinomas occurred in both species. In medaka, the lesions appeared to develop sequentially with the appearance of altered foci followed by adenomas and then hepatocellular carcinomas. Most lesions in guppies, however, were classified as altered foci though a few adenomas occurred in the early (24-week) sample and hepatocellular carcinomas occurred in the late (52-week) sample. When total lesions were combined, medaka had an 11% incidence at 24 weeks after the initial exposure and 36% incidence at 36 weeks. In guppies, 8% had liver lesions at 24 weeks, 19% at 36 weeks, and 20% at 52 weeks. A single extrahepatic neoplasm, a capillary hemangioma in a gill filament, occurred in a medaka from the 36-week high-dose sample. The results suggest that the medaka and guppy are capable of metabolizing water-borne BaP to carcinogenic metabolites which initiate hepatic tumor development.