Rates and patterns of scnDNA and mtDNA divergence within the Drosophila melanogaster subgroup.
Rates and patterns of scnDNA and mtDNA divergence within the Drosophila melanogaster subgroup.
复制标题
果蝇亚群内 scnDNA 和 mtDNA 的分化率和模式。
DOI：
10.1093/genetics/118.4.671
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发表时间：
1988
期刊：
影响因子：
3.3
通讯作者：
Powell,JR
中科院分区：
文献类型：
--
作者：
Caccone,A;Amato,GD;Powell,JR
用DNA-DNA杂交技术测定了黑腹果蝇亚群的8种果蝇和高氏果蝇的DNA差异水平。使用两种类型的DNA：单拷贝核DNA(ScnDNA)和线粒体DNA(MtDNA)。主要发现如下：(1)基于scnDNA的群体系统发育与染色体数据、形态和行为相一致。Simans、sechellia和Mauritiana这三个同序种的亲缘关系非常密切；scnDNA的差异表明这两个岛种是一个单系群。(2)scnDNA和mtDNA的变化率差异不大，如果有什么不同的话，scnDNA的进化速度比mtDNA快。(3)scnDNA进化速率与染色体倒位进化关系不密切。(4)果蝇基因组似乎由两种不同的scnDNA组成，一种是进化速度非常快的scnDNA，另一种是相对保守的scnDNA。(5)绝对变化率估计为每百万年至少有1.7%的核苷酸替换。(6)基于限制性内切酶切点变异的DNA距离估计与基于DNA-DNA杂交的距离是相关的，尽管相关性不是很强。
Levels of DNA divergence among the eight species of the Drosophila melanogaster subgroup and D. takahashii have been determined using the technique of DNA-DNA hybridization. Two types of DNA were used: single-copy nuclear DNA (scnDNA) and mitochondrial DNA (mtDNA). The major findings are: (1) A phylogeny has been derived for the group based on scnDNA which is congruent with chromosomal data, morphology, and behavior. The three homosequential species, simulans, sechellia, and mauritiana, are very closely related; the scnDNA divergence indicate the two island species are a monophyletic group. (2) The rates of change of scnDNA and mtDNA are not greatly different; if anything scnDNA evolves faster than mtDNA. (3) The rates of scnDNA evolution are not closely correlated to chromosomal (inversion) evolution. (4) The Drosophila genome appears to consist of two distinct classes of scnDNA with respect to rate of evolutionary change, a very rapidly evolving fraction and a relatively conservative fraction. (5) The absolute rate of change was estimated to be at least 1.7% nucleotide substitution per one million years. (6) DNA distance estimates based on restriction site variation are correlated with distances based on DNA-DNA hybridization, although the correlation is not very strong.