Evolution of Resistance in Culex quinquefasciatus (Say) Selected With a Recombinant Bacillus thuringiensis Strain-Producing Cyt1Aa and Cry11Ba, and the Binary Toxin, Bin, From Lysinibacillus sphaericus.
Evolution of Resistance in Culex quinquefasciatus (Say) Selected With a Recombinant Bacillus thuringiensis Strain-Producing Cyt1Aa and Cry11Ba, and the Binary Toxin, Bin, From Lysinibacillus sphaericus.
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用产生 Cyt1Aa 和 Cry11Ba 的重组苏云金芽孢杆菌菌株以及来自球形赖氨酸芽孢杆菌的二元毒素 Bin 筛选出的致倦库蚊（Say）的耐药性进化。
DOI：
10.1093/jme/tjv115
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发表时间：
2015
影响因子：
2.1
通讯作者：
Federici,BrianA
中科院分区：
文献类型：
--
作者：
Wirth,MargaretC;Walton,WilliamE;Federici,BrianA
Fourth instars ofCulex quinquefasciatus(Say) (Diptera: Culicidae) were selected with a recombinant bacterial strain synthesizing the mosquitocidal proteins fromLysinibacillus sphaericus(Bin) and Cry11Ba and Cyt1Aa fromBacillus thuringiensis. Selection was initiated in Generation 1 with a concentration of 0.04 μg/ml, which rose to a maximum selection concentration of 8.0 μg/ml in Generation 14, followed by an unexpected, rapid increase in mortality in Generation 15. Subsequently, a selection concentration of 0.8 μg/ml was determined to be survivable. During this same period, resistance rose to nearly 1,000-fold (by Generation 12) and declined to 18.8-fold in Generation 19. Resistance remained low and fluctuated between 5.3 and 7.3 up to Generation 66. The cross-resistance patterns and interactions among the component proteins were analyzed to identify possible causes of this unusual pattern of evolution. Poor activity in the mid-range concentrations and lower-than-expected synergistic interactions were identified as potential sources of the early resistance. These findings should be considered in the development of genetically engineered strains intended to control nuisance and vector mosquitoes.
